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Objective:To study the distribution of CD14 in human gingival fibroblasts (HGFs) and the effects of lipopolysaccharide(LPS) on mCD14 expression in HGFs in vitro. Methods:HGFs were in vitro cultured and exposed to LPS at 0.1-10 ?g/L.The expression of mCD14 in the cells was examined by immunohistochemistry staining and Western blot.Results:mCD14 was found on healthy human HGF cell membrane surface and LPS at 1 ?g/ml and 10 ?g/ml increased the expression of mCD14 in 1-12 h exposure.Conclusion:LPS may regulate mCD14 expression of HGFs.
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Objective:To prepare a rabbit anti-mouse Periostin IgG antibody and to study the expression of Periostin in mice.Methods:Complete Freund adjuvant with recombinant Periostin was used to immunize rabbits.Whole blood was collected from the animals after immunization for 5 times. Serum was obtained by centrifugation. The specific IgG antibody was purified from the serum by ammonium sulfate. Total protein isolated from mouse liver, brain, kidney and calvaria and periodontal tissue was used to study the expression of Periostin in mice by Western blot, immunohistochemical staining was applied to examine Periostin expression in jaw bone and dental tissues.Results:Anti-Periostin antibody with the titer of 128 was obtained.Western blot showed that there was a specific band near Mr 90 000 in calvaria and periodontal tissues, but not in liver, brain and kidney.By immunohistochemical examination Periostin was detected in both mandibular periosteum and periodontal ligament,but not in cementum, dentin, alveolar bone and dental pulp.Conclusion:Periostin may play a role in bone and periodontal tissue formation.
RESUMO
Objective: To observe the effects of human insuline like growth factor 1 (rhIGF 1) and/or basic fibroblast growth factor (bFGF) on the proliferation of periodontal ligament (PDL) cells. Methods: PDL cells were exposed to rhIGF 1 and/or bFGF at various concentrations for 1,3,5 and 7 d respectively. The proliferation of the cells was studied with MTT assay.Results: rhIGF 1 at 3.125～25.000 ?g/L or bFGF at 0.1～10 ?g/L promoted PDL cells growth in a dose and time dependant way.3.125 ?g/L of rhIGF 1 and 1 ?g/L of bFGF showed stronger effects on cell growth than both used individually.Conclusion: rhIGF 1 and/or bFGF can promote the proliferation of PDL cells.