diagnostic efficacy of Fasciola gigantica coproantigen in naturally infected cattle and buffaloes

Monoclonal antibody sandwich ELISA was used detect F. gigantica coproantigen in 33 selected animals according to the faecal history to evaluate sensitivity and specificity by using three different rabbit hyper-immune sera; Copro HIS, Egg HIS and ES HIS. The results showed that Copro HIS and ES HIS detected F. gigantica coproantigen in faecal of naturally infected cattle and buffaloes, but egg HIS failed. The 26-28 KDa coproantigen proved sensitivity [81.8%] and specificity [90.9%] in diagnosis of fascioliasis. Also, there was a positive statistical significance between number of F. gigantica egg per gm faeces [EPG] and mean sandwich ELISA OD. values for copro antigen. For diagnostic value of F. gigantica coproantigen in comparison with ES antigen, EITB was done on field sera of cattle and buffaloes of known faecal history. The F. gigantica coproantigen bands of 27.6 and 72.1 KDa were specific for diagnosis animal fascioliasis, but the 72.1 KDa was less sensitive than the 27.6 KDs. The immunoblotting reaction was more intensive than with fractionated ES antigen than with fractionated coproantigen

Efficacy of some Fasciola gigantica antigens

The present study tested the antigenic relationship between the three Egyptian strain of Fasciola gigantica antigens; coproantigen, excretory-secretory and egg antigens, versus their related hyper-immune sera and select the most specific one. By using SDS-PAGE, a structural homology was demonstrated in F. gigantica ES and egg antigens. This homology was resided in the components of the similar molecular weights between both antigens. When no cross-reaction was recorded with the coproantigen, the intense cross-reaction occurred between ES and egg antigens in ELISA technique. This was attributed to the presence of common bands at 18.0, 20.4 and 27.6 KDa in between them. Consequently, the F. gigantica coproantigen and Copro HIS reflected the lowest level of the cross-reaction with the other evaluated F. gigantica antigens. The cross-reaction elucidated in the present study between the F. gigantica ES and egg antigens was mainly at the low serum dilutions. The distinction between the specific and the cross-reactive binding activities was clearly marked with the highly diluted sera

Anti-fasciola antibodies among rodents and sheep in Jeddah, Saudi Arabia

A total of 20 imported sheep were examined for fascioliasis natural infection by kato thick smear and by Fasciola-indirect haemagglutination test [IHAT]. Stool examination revealed infection in 13/20 [65%], but IHAT identified 11/20 [55%]. So, the sensitivity was 84.5%. Also, five species of rodents trapped within or nearby the infected sheep farm by IHAT showed positive reactions of in Rattus rattus [22.4%], R. norvegicus [16.7%], Meriones crassus [11.8%], Juculus jaculus [zero%], Acomys c. dimidiatus [9.4%], The overall positive IHA reaction was 11/143 or 14.7%. The presence of natural fascioliasis infection in imported sheep and wild rodents were discussed