RESUMO
HTLV-I seroprevalences of 3.63% (02/55), 12.19% (10/82) and 13.88% (10/72) were demonstrated among Tiryio, Mekranoiti and Xicrin Amazonian Indians, respectively, by the Western blotting enzyme assay (WBEI). By indirect immunoelectron microscopy (IIEM), 2 Tiriyo, 9 Mekranoiti and 6 Xicrin Amerindians were reactive. Of 44 serum samples from Japanese immigrants, none reacted by any of the techniques before mentioned. One, 8 and 6 serum samples from Tiryio, Mekranoiti and Xicrin Indians, respectively, were both WBEI and IIEM positive. Our results strongly suggest that HTLV-I and/or an HTLV-I antigenic variant circulate (s) among populations living in the Amazon region of Brazil
Assuntos
Humanos , Anticorpos Anti-HTLV-I/sangue , Infecções por HTLV-I/etnologia , Infecções por HTLV-I/imunologia , Indígenas Sul-Americanos , Havaiano Nativo ou Outro Ilhéu do Pacífico , Western Blotting , Brasil/epidemiologia , Imuno-Histoquímica , Indígenas Sul-Americanos/estatística & dados numéricos , Japão/etnologia , Microscopia Imunoeletrônica , Havaiano Nativo ou Outro Ilhéu do Pacífico/estatística & dados numéricos , Prevalência , Estudos SoroepidemiológicosRESUMO
Amostra de soro obtida de paciente com a sindrome de Guillain-Barre revelou-se positiva quanto a presenca de anticorpos para o virus linfotropico humano T (HTLV-I) pelo metodo imuno-enzimatico (ELISA) e a analise por "Western-Blot". Resultaram negativos os testes visando a deteccao de enterovirus (incluindo poliovirus) a partir de material fecal, tanto em cultura de tecidos como em camundongos recem-nascidos; exames com amostras de soro aguda e convalescente nao exibiram qualquer evidencia de infeccao recente pelos tres tipos de poliovirus. O teste de Paul-Bunnel, assim como o "ELISA" para a deteccao de IgM anti-citomegalovirus resultaram negativos. Nao foi registrada, no presente caso, quer a leucemia adulta de celulas T, quer linfomas.
Assuntos
Criança , Humanos , Masculino , Anticorpos Anti-HTLV-I/análise , Polirradiculoneuropatia/imunologia , Western Blotting , Ensaio de Imunoadsorção EnzimáticaRESUMO
Forty-tree (31.4%) out of 137 serum samples obtained from two Indian communities living in the Amazon region were found to be positive for HTLV-I antibody, as tested by enzyme-linked immunosorbent assay (Elisa). Eighty-two sera were collected from Mekranoiti Indians, yielding 39% of positivity, whereas 11 (20.0%) or the 55 Tiriyo serum samples had antibody to HTLV-I. In addition, positive results occurred in 10 (23.2%) out of 43 sera obtained from patients living in the Belem area, who were suffering from cancer affecting different organs. Five (16.7%) out of 30 Elisa positive specimens were also shown to be positive by either Western blot analysis (WB) or indirect immunogold electron microscopy (IIG-EM)