Trend of Low Serum Vitamin B[12] in Jordanian Adults from Two Ethnic Groups in Amman

To investigate the prevalence of vitamin B[12] deficiency in Jordanian adults from two ethnic groups in Amman. The plasma B[12] level was measured in 290 subjects [124 men, 166 women, aged 16-72] from two ethnic groups; Circassians and Arabs. These participants were recruited by simple random sampling from both communities from June 2004 to March 2005. The prevalence of vitamin B[12] deficiency [B[12] level lower than 200 pg/ ml] was 50.8 and 46.9% in Arab and Circassians, respectively. No significant differences in the plasma B[12] level were found between the different age groups in Arabs and Circassians and between the same age groups of the two populations. One notable exception was the subjects who are older than 60 years in Circassians. Their plasma B[12] levels were significantly lower than those in the other age groups in Circassians and the same age group in Arab [P<0.05]. There were differences in the plasma B[12] level according to gender; the plasma B[12] levels for men were lower than those obtained for women in both groups. This difference was significant [P<0.001] for Arab population. Compared to Circassian men, the plasma B[12] levels of Arab men were also significantly lower [P< 0.001]. The plasma B[12] level was significantly lower in smokers compared to non-smokers in both groups [P<0.005]. The current study demonstrates the high prevalence of suboptimal cobalamin levels in Jordanian subjects living in Amman, and it shows that there are no differences in the prevalence as related to the two ethnic groups studied

primer of an animal cell culture laboratory for efficacy, toxicity and transport screening

In the advent of the discovery of new molecules with potential therapeutic effect and the engineering of new drug delivery systems, the availability of in vitro test systems for efficacy, transport and toxicity screening are becoming of an increasing importance. In this report, we present the initiation of a cell culture system for the above mentioned purposes. The report describes some of the validated assays implemented and preliminary studies on toxicity and efficacy