Transmissible drug resistance among infantile enteropathogenic escherichia coli strains and urinary non lactose fermenting E coli strains isolated in Alexandria, Egypt

As a part of surveillance of drug resistance in eterobacteria in Alexandria, Egypt, two groups of E. coli were selected: 20 EPEC strains isolated from the stools of diarrheal infants and 11 strains of NLF E. coli [atypical E. coli] isolated from urine of outpatients suffering from persistent urinary tract infection. Antibiotic sensitivity testing revealed that 80% of EPEC and 72% of NLF E. coli were resistant to more than two antibiotics. 62.5% of the resistant EPEC were able to transfer resistance to various degrees to the standard recipient and the commonest pattern transferred was AM C S N SD; only one strain of NLF E. coli initially resistant to AM C N S SD TE SXT was able to transfer resistance to AM C N TE to the standard recipient

Isolation, biotyping and sugar fermentation reactions of haemophilus influenzae biotype II and biotype III strains isolated from cases of conjunctivitis in Beheira - Egypt

Among Hemophilus both H. egypticus and H. influenzae biotype III share the etiology of conjunctivitis in Egypt. They also share the same key biochemical characteristics. Consequently, it was felt necessary to determine de novo the action of Hemophilus biotype III on a large number of sugars in order to verify the assumed resemblance or perhaps reveal more differential characters. Seventy strains of Hemophilus influenzae were isolated from cases of acute mucopurulent conjunctivitis in Behaira. Biotyping differentiated the strains into 50 strains of H. influenzae biotype III and 20 strains of biotype II. All strains were tested for acidification of 22 sugars. Fermentation could only be detected in 11 sugars

Evaluation of netilmicin therapy in patients with severe urinary tract infection

This study comprised 25 patients admitted to Gamal Abdel-Nasser Hospital in Alexandria with severe urinary tract infection as evidenced by heavy growth of bacteria [Gram-negative bacilli and Staph. aureus] from cultures of urinary specimens. The strains were tested for antibiotic susceptibility by the single disc diffusion technique and all strains proved to be multiresistant, but sensitive to netilmicin. These patients were of normal renal function and were therefore subjected to a therapeutic trial using netilmicin sulfate. 300 mg of netilmicin were given daily in two divided doses by the intramuscular route for 7-10 days. The peak and trough serum concentrations of netilmicin were measured on the second and seventh days of therapy and the renal function was assessed by estimation of creatinine level before and after therapy. Complete or marked improvement of signs and symptoms and eradication of the causative pathogens were observed in 22/25 [88%] of the patients. The three patients that failed to recover both clinically and bacteriologically were those who had multiple urinary stones

association of chlamydia trachomatis and herpes simplex with male urethritis

The association of HSV and Chlamydia trachomatis with urethritis was determined by isolation procedures applied to 100 male patients suffering from urethritis and attending the Bacous Venereal Diseases Clinic. Three swabs are collected from each case; one to inoculate Thrayedr-Martin medium, the second to inoculate the collection medium to be used for isolation of Chlamydia trachomatis by inoculation of McCoy cell line treated with cyclohexamide, and the third to inoculate the collection medium to be used for isolation of HSV by chorio allantoic membrane inoculation. Chlamydia trachomatis was recovered from 33.8% of NGU and 8.6% of the gonococcal cases. HSV was recovered from 7.8% of NGU and 4.3% of the gonococcal cases. All HSV was of type II [by neutralization method]