RESUMO
This study was carried out to evaluate the antimicrobial and antioxidant effectiveness of methanolic extract and different fractions [n-butanol, ethyl acetate, chloroform and n-hexane] of C.intybus seeds. The antimicrobial activity was determined by the disc diffusion method and minimum inhibitory concentration [MIC] against a panel of microorganisms [four bacterial strains, i.e. P. multocida, E. coli, B. subtilis and S. aureus and three fungal strains, i.e A. flavus, A. niger and R. solani]. The results indicated that seeds extract and fractions of C. intybus showed moderate activity as antibacterial agent. While Antifungal activity of C. intybus seeds extract/fractions was very low against A. flavus and A. niger while mild against R. solani. The C.intybus seeds extract/fractions contained appreciable levels of total phenolic contents [50.8-285 GAE mg/100g of Dry plant matter] and total flavonoid contents [43.3-150 CE mg/100g of Dry plant matter]. The C. intybus seed extract/fractions also exhibited good DPPH radical scavenging activity, with IC[50] ranging from 21.28-72.14 micro g/mL. Of the C .intybus seeds solvent extract/fractions tested, 100% methanolic extract and ethylacetate fraction exhibited the maximum antioxidant activity. The results of the present investigation demonstrated significant [p < 0.01] variations in the antioxidant and antimicrobial activities of C. intybus seeds solvent extract/fractions