RESUMO
Screening of different extracts and fractions from the plant Bidens pilosa check for this species in other resources Linn. var. (Asteraceae) has been conducted using the in - vitro comet assay for anticancer and the antipyretic action; which was done with in - vivo models.The extract from whole plant was extracted with n - hexane;chloroform and methanol extract (E1 - E3).The extracts were fractioned by column chromatography method and fractioned with ethyl acetate; acetone and water (F1 - F3).All the extracts and fractions were tested for anticancer and antipyretic activity. Among extracts E1 shows remarkable anticancer activity and E3 bears maximum antipyretic activity. In the antipyretic activity; paracetamol was used as the standard test drug.The most promising material (LC50 1500 ?g / ml) was F1 ethyl acetate fractions of methanolic extract and methanolic crude extract of whole plants. However; little correlation was observed in the degree of antipyretic activity between the test drug and standard drug. In conclusion; the extract obtained from the whole plant of Bidens pilosa showed a significant cytotoxic effect to methanolic extract against Hela cells by in vitro method and showed a comparable antipyretic activity effect to paracetamol in rabbit pyrogen test