effects of different storage media on the regeneration of pdl in experimentally avulsed teeth [histological and immuno-histochemical study]

The choice of storage medium for preserving the traumatically avulsed teeth is important for the success of future replantation. The objectives of this study were to evaluate the effectiveness of pre-replantation storage media on periodontal ligament healing. Extracted, fully-developed rat maxillary incisors were stored in milk, saline or tap water for 15 minutes. Teeth that had been stored in either cold milk or saline showed regeneration of typical PDL, while those stored in tap water showed signs of degeneration. The present study has showed that cold milk and saline may thus be rec-'ommended as storage medium for ex-articulated teeth prior to replantation in case when immediate replantation is not possible

Scanning electron microscopic observations of the root surface in both permanent and deciduous teeth

This study describes the histological features of human cementum along different locations of the root in both deciduous and permanent teeth. 20 freshly extracted teeth with minimal periodontal involvement were collected. They include deciduous [anterior and posterior] teeth and permanent [anterior and posterior] teeth. Scanning electron microscopic observations revealed presence of minute resorping lacunae in both deciduous and permanent teeth with great variability in the number and depth. A striking observation revealed the presence of cracks on the root surface of permanent teeth and their absence in deciduous one. The recorded differences may be related to the differences in the fate as well as the function of the roots in both dentitions. Where in deciduous teeth, roots were re-sorped during shedding whereas in permanent teeth, cementum provides media for the attachment of periodontal ligament through out the life span of the tooth

Histological, histochemical and immunohistochemical demonstration of the effect of certain commonly used diuretics on rat tongue epithelium

The purpose of this study aimed to investigate the effect of certain commonly used diuretics as furosemide, amiloride and acetazolamide on the rat tongue epithelium. The study was conducted on 20 adult male rats, divided equally into 4 groups. Control group; group I: received furosemide in a daily oral dose of 40 mg/Kg; group II: received amiloride in a daily oral dose of 4mg/Kg; and group III: received acetazolamide in a daily oral dose of 8mg/Kg. Histological, histochemical and immunohistochemical study were carried out revealing that the three experimental groups demonstrated significant epithelial hyperplasia and alterations in the tongue epithelium structures. The degree of changes proved to be more severe in group I followed by group II.and then group III. These deteriorative changes may suggest increased enzymatic activity and general disturbance in the cell metabolism and homeostasis