possible role of Petroselinum Sativum extract against oxidative stress in CCL[4]-induced hepatotoxicity in albino rats

The present study investigated the possible effect of the ethanolic extract of Petroselinum sativum against CCl[4]-induced hepatotoxicity in rats and compared with Rutin as reference. Ethanolic extract of Petroselinum sativum in two concentrations 100 and 200 mg/kg.bw. Was orally administered for 21 days after i.p CCl[4] injection to evaluate its possible effect. Also, Rutin [as standard antioxidant] was orally administered by concentration of 200 mg/kg.bw. Malondialdehyde "MDA" concentration [the end product of lipid peroxide] and total protein were determined both in liver, homogenate and plasma. Reduced Glutathione "GSH" content was determined in liver homogenate and in whole blood. Albumin was also determined and Glutathione-S-transferase "GST" in serum of rats was performed. Histological studies of liver for all treatments were investigated and results revealed that, the lipid peroxides "MDA" and "GST" were significantly increased [p<0.05], while reduced glutathione was decreased after treatment of CCL[4]. Administration of Petroselinum Sativum ethanolic extract at two concentrations and Rutin exerted a significant decreases [p<0.05] in MDA concentration, while the reduced glutathione appeared to be increased in whole blood. The activity of GST was reduced due to the effect of Petroselinum Sativum and Rutin after the duration time [10 and 21 days]. The ethanolic extract of Petroselinum Sativum revealed a significant increase in the total protein, albumin, and A/G ratio. The histological studies showed a significant increase in liver injury of intoxicated rats. After treatment with ethanolic extract of Petroselinum Sativum as well as Rutin, the sites of injury were decreased. It was concluded that, the toxic effect of CCl[4] on liver was abrogated under the effect of Petroselinum Sativum ethanolic extract; also, Rutin had an inhibitory effect on lipid peroxidation. These findings suggested that the plant extract of Petroselinum Sativum could has a potent antioxidant activity

Comparative effect of petroselinum sativum extract and rutin on liver functions

Serum enzymes are very important in clinical diagnosis of diseases and estimation of liver damage of intoxicated rats by CCL[4]. In this study the effect of Petroselinum sativwn extracts was measured on the enzyme activities of liver transaminase "ALT, AST, ALP, and LDH". Also, the activity of lysosomal enzymes "ACP; beta-GAL, and beta-NAG In addition to superoxide dismutase as an antioxidant enzyme were determined after oral treatment with two concentrations "50 and 100 mg/kg b.w" for 21 days after injection i.p. of CCL[4] to evaluate the possible curative effect for the ethanolic extract of parsley. The activity of SOD as an antioxidant enzyme was also studied. Rutin as a standard antioxidant was orally administered as single dose "100 mg/kg b.w" for the same periods "10, and 20 days". This experiment was performed in two groups of rats "intoxicated and nontoxicated treatments". The results revealed that the liver enzymes activity "ALT, AST, ALP, and LDH" were markedly increased in hepatotoxic rats after the duration time. The higher activities of these enzymes could be referred to induced cell damage after CCL[4] treatment. Also, the lysosomal enzymes such as "ACP, beta-GAL, and beta-NAG" were markedly increased in parallel to the hepatoxic rats, SOD activity was significantly decreased. After plant extract administration by the two concentrations and Rutin by single dose, the enzyme activities of "ALT, AST, ALP, LDH, ACP, beta-GAL, and beta-NAG" were ameliorated and decreased significantly as compared to the control group. SOD activity was increased. Petroselinum sativum ethanolic extract exerted a curative effect on the enzyme activities of transaminase, "ALP, LDH, and the activities of lysosomal enzymes of rat liver. Also, this extract suppressed the oxidative stress by enhancement of the SOD activity

Effect of interferon alfa-2-beta on the DNA, RNA, proteins and the O[2]-uptake of Rat liver mitochondria and homogenates

The effect of interferon alfa-2-b [IFN] on O[2]-uptake of isolated rat liver homogenate and mitochondria at 37°C over two hours were examined in two concentrations "6428.5 and 3214.3 IU/kg rat". The results of the rates of aerobic respiration of rat liver homogenate revealed the occurrence of uniform reductions in total O[2]-consumption after 1, 2, 3 and 4 hours exposure to the two concentrations of interferon. On the other hand, the O2-uptake of mitochondria exerted an enhancement effect after the exposure time under the effect of the drug. Protein patterns were determined and identified by SDS/PAGE method, genomic DNA was extracted and purified, then isolated on 1% agarose for analysis the molecular size of DNA after treatment with interferon by the two doses. RNA was extracted and purified and then isolated on agarose 1.3% for determining the differences in the molecular size between the treated and nontreated samples. The protein concentration was significantly increased by the two doses of the drug. The protein patterns on SDS/PAGE revealed different bands with different molecular Daltons. The genomic DNA isolated from rat liver mitochondira or homogenate have a high molecular size [more than 1.0 kbp.]. In addition, interferon at the low dose decreased the RNA concentration either in rat liver homogenate or mitochondria, while the high dose of the drug increased the concentration of RNA. The molecular weight of isolated RNA on agarose either from rat liver homogenate or mitochondria after drug treatment exhibited a molecular size more than 1031 bp. PCR amplification was conducted on genomic DNA of rat liver homogenate after interferon alpha 2-b treatment by the high dose only. All PCR products appeared to be identical and the bands had the same molecular weights