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1.
Mycobiology ; : 52-56, 2017.
Artigo em Inglês | WPRIM | ID: wpr-729887

RESUMO

In this study, we report the manganese peroxidase production ability from a Fusarium sp. strain using an inexpensive medium of agriculture residues of either rice straw or wood chips as carbon source. The highest manganese peroxidase activity on rice straw medium and on wood chips was 1.76 U/mL by day 9 and 1.91 U/mL by day 12, respectively.


Assuntos
Agricultura , Carbono , Fusarium , Manganês , Programas de Rastreamento , Peroxidase , Madeira
2.
Mycobiology ; : 121-124, 2011.
Artigo em Inglês | WPRIM | ID: wpr-729398

RESUMO

The cDNA of endo-1,4-beta-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the alpha-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively.


Assuntos
Chrysosporium , DNA Complementar , Phanerochaete , Pichia , Polissacarídeos , Sinais Direcionadores de Proteínas , Saccharomyces cerevisiae
3.
Mycobiology ; : 182-186, 2011.
Artigo em Inglês | WPRIM | ID: wpr-729387

RESUMO

Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.


Assuntos
Ágar , Sequência de Aminoácidos , Quitina , Quitinases , Coloides , Peso Molecular , Solo , Entorses e Distensões , Trichoderma , Vietnã
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