Percentage Body Fat is As a Good Indicator for Determining Adolescents Who Are Overweight or Obese: A Cross-Sectional Study in Vietnam

OBJECTIVES: To identify trends in percentage body fat (PBF) in adolescents to determine gender-specific classification thresholds for being overweight and obese. METHODS: A cross-sectional study of 899 adolescents (11 to 14.5 years) from Hanoi and Nam Dinh was conducted in Vietnam. PBF, subcutaneous fat and percentage of lean mass were measured directly using OMRON HBF 375 scales to measure bioelectrical impedance. RESULTS: PBF decreased in boys with increasing age (p < 0.001). The difference in PBF between boys and girls, significantly increased with age after 12.5 years (p < 0.001). There was a stronger correlation between PBF and fat content (Pearson's r = 0.860, p < 0.0001) than that between (BMI) and fat content (Pearson's r = 0.521, p < 0.0001). The prevalence of being overweight or obese in girls was similar when determined by PBF or BMI. Hanoi had higher rates of adolescents being overweight or obese compared with Nam Dinh. CONCLUSION: PBF provides a more accurate body assessment for being overweight or obese in adolescents compared with BMI.

Childhood Obesity Is a High-risk Factor for Hypertriglyceridemia: A Case-control Study in Vietnam

OBJECTIVES: To investigate the relationship between dyslipidemia and obesity status among Viet-namese adolescents. METHODS: In this case-control study, 282 adolescents (6–11 years), including 88 obese cases and 194 normal-weight controls, were recruited from a population-based cross-sectional study from two provinces in Vietnam. The anthropometric, blood lipid, and other laboratory test results of the study subjects were analyzed. RESULTS: Obese children tended to have more visceral fat (Pearson's r = 0.795, p < 0.0001) than subcutaneous fat (Pearson's r = 0.754, p < 0.0001), and this difference was associated with an increase in blood triglyceride level (Pearson's r = 0.232, p < 0.05) and a strikingly high rate of hypertriglyceridemia (38.6%). We also found that birth weight and parental body mass index were related to the status of obesity among the study subjects. However, only birth weight was significantly higher in the obese group than in the normal weight group. These findings indicate the effect of prenatal nutrition on childhood obesity. Furthermore, high-birth weight children had a surprisingly high rate of obesity. CONCLUSION: Together, our data suggest that obesity increased the risk for hypertriglyceridemia, which was, at least partially, due to prenatal nutrition.

Study on the technology to produce inactivated rabies vaccine on the primary hamster kidney cell at laboratory scale

Vnukovo-32 a cell culture rabies is used to study the vaccine strain technology of inactivated culture rabies vaccine production in primary hamster kidney cells. The master and working seeds meet the criteria of rabies vaccine strain titer (5.5 log LD 50 - 7.1 log LD 50/ml) according to WHO standard. During the study, we have used the procedure for inactivated, non concentrated rabies vaccine production of Russia and other countries procedures as references. The result was we constructed the procedure for inactivated, concentrated cell culture rabies vaccine production at laboratory scale.

The use of the recombinant protein HP1125 for detecting helicobacter pylori

The outer membrane protein HP 1125 of Helicobacter pylori was expressed as a recombinant protein in E.Coli by a short time method for Western and ELISA analysis. The analysis was conducted on more than 100 patients in Vietnam

Detection of Leishmania SP. by PCR method

There is a conservative region of more than 130 bp long in the mitochondria genome of Leishmania mexicana M379 clone E8, and this it is found across many other Leishmania spp. Based on this notion, a pair of PCR primers were designed to amplify the corresponding regions in the minicircles of many other Leishmania species such as L.major, L.donovani. PCR method can be used to detect protozoa in Vietnam