RESUMO
Background: Fungal keratitis is a serious ocular infection that can cause corneal scarring and blindness. Currently, diagnosis of fungal pathogens remains a difficult problem. Objectives: To investigate the application of semi-nested PCR targeted ITS genes for detection of fungal agents causing keratitis. Material and method: Ten identified fungal strains, 4 bacterial strains, 20 scraping samples from patients with suspected fungal keratitis and 2 scraping samples from patients with suspected bacterial keratitis were tested using semi-nested PCR. Results: Semi-nested PCR showed positive results for the samples of identified fungal strains and for the 20 scraping samples from patients with suspected fungal keratitis. Neither samples of bacterial strains nor scraping samples from suspected bacterial keratitis patients gave positive PCR results. Conclusion: Semi-nested PCR is a robust tool for specific and rapid detection of fungal agents causing keratitis.
RESUMO
Background:blaTEM, bla SHV, blaCTX \ufffd?M, blaOXA genes encode for extended spectrum \u03b2 \ufffd?lactamases resistance to broad \ufffd?spectrumcephalosporins. Many species belonging the family Enterobacteriaceae possess these genes. Objectives: To determine the distribution of blaTEM, bla SHV, blaCTX \ufffd?M and blaOXA genes in enteroaggregation E.coli (EAEC) strains. Subjects and method: 67 EAEC strains causing diarrhea and 18 strains isolated from healthy children were screened by PCR with primers specific to blaTEM, bla SHV, blaCTX \ufffd?M \ufffd?1and blaOXA genes. Results: The prevalence of ESBL genes in diarrheagenic EAEC strains and those isolated from healthy children were 83.6 and 72.2 %, respectively. The highest prevalence blaTEM gene (82% in diarrheagenic EAEC strains and 72.2% in isolated from healthy children) was followed by that of blaOXA gene (11.9 and 11.1% in two EAEC groups). Only 2 strains possess blaSHV gene. The blaCTX \ufffd?M \ufffd?1 was not detected in any EAEC strain. Conclusion: our findings have not only provided additional understanding of the distribution of blaTEM, bla SHV, blaCTX \ufffd?M - 1 and blaOXA genes in EAEC strains but also have a given significance in selecting antibiotics for treatment.