RESUMO
Background: PCR technique combined with culture isolates is used commonly to detect bacteria that can cause diarrhea from clinical samples. Direct DNA extraction which is a simple, fast and effective method is very important in the rapid diagnosis of bacterial pathogens from fecal samples. Objective: Perfection of method for direct DNA extraction of Shigella and EIEC from fecal samples. Subjects and method: EIEC ATCC 43893, E. coli ATCC 11775 was used for positive and negative controls. Extracted DNA would be used for PCR. Results: The method for direct DNA extraction of Shigella and EIEC from fecal sample had been successfully developed. It was a simple, rapid, cheap and applicable method. Conclusion: The method for direct Shigella/EIEC DNA extraction had been successfully developed and it was very helpful in early detection of diarrheal-induced bacterial pathogens.