RESUMO
Objective: To determine the effect of glucose, sucrose, and saccharin on growth, adhesion, and biofilm formation of Candida albicans and Candida tropicalis
Materials and Methods: The growth rates of mono-cultures of planktonic C. albicans and C. tropicalis and 1:1 mixed co-cultures were determined in yeast nitrogen broth supplemented with 5% [30 mM] and 10% [60 ITIM] glucose, sucrose, and saccharin, using optical density measurements at 2-h intervals over a 14-h period. Adhesion and biofilm growth were performed and the growth quantified using a standard 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide [MTT] assay. The biofilm architecture was visualized using scanning electron microscopy. One- and two-way analysis of variance [ANOVA] was performed to analyse the differences among multiple means
Results:The highest planktonic growth was noted in 5% glucose after 14 h [p < 0.05]. No significant planktonic growth was observed in either concentration of saccharin. Both the concentrations of glucose and sucrose elicited significantly increased adhesion from MTT activity of 0.017 to >0.019 in mono- as well as co-cultures [p < 0.05], whilst the lower concentration of saccharin significantly dampened the adhesion. Maximal biofilm growth was ob-served in both species with the lower concentration of sucrose [5%], although a similar concentration of saccharin abrogated biofilm development: the highest MTT value [>0.35] was obtained for glucose and the lowest [>0.15] for saccharin
Conclusion: In this study, glucose and sucrose accelerated the growth, adhesion, and biofilm formation of Candida species. However, the non-nutritive sweetener saccharin appeared to dampen, and in some instances suppress, these virulent attributes of Candida