RESUMO
To see the serotyping and antibiotic sensitivity by MIC of Pseudomonas Aeruginosa strains Design: The serotyping was done by agglutination method and MIC was determined by microdilution method, according to NCCLS guidelines. MIC of Amikacin, Aztreonam, Ceftazidime, Cefotaxime, Gentamicin, Imipenem, Ofloxacin and Piperacillin was determined. The ATCC strains of Pseudomonas Aeruginosa No. 27857 was used as quality control. Setting: Patients were admitted in Kumamoto University Hospital, Japan. Prospective study involving 78 strains of Pseudomonas aeruginosa isolated from patients admitted in different wards. Main Outcome Measures: Serotyping and MIC of isolated Pseudomonas aeruginosa strains against 8 antibiotic drugs of different groups. The serotypes of isolated strains were B 18 [23.07%], I 16 [20.51%], Non 14 [17.94%], G 11 [14.10%] E 9 [11.53%], A 3 [3.84%], C 3 [3.84%], D 3 [3.84%] and F 1 [1.28%]. Pseudomonas aeruginosa strains were isolated from all types of specimens, but majority was from infected wounds [Pus] and urine. Susceptibility to Amikacin was 89.75% while Aztreonam and Imipenem was 88.47%. The isolates were moderately susceptible to Ceftazidime [85.90%], Piperacillin [84.47%] and Gentamicin [83.34%], while they were resistant to Ofloxacin in 26.92% and Cefotaxime in 37.17% cases. Serotyping is a useful indicator for the phenotypic characterization of Pseudomonas aeruginosa. Pseudomonas aeruginosa is highly susceptible to Amikacin while resistant Ofloxacin and Cefotaxime Serotype B was noted as common in urine specimen showing multi-resistance to antimicrobial drugs