RESUMO
We describe a "sandwich" enzyme-linkedimmunosorbent assay (ELISA) sensitive to quantities of scorpion (Tityus serrulatus) venom (TsV) in the range of 1-3 ng?ml sample. Cross-reactivity with the venom from the rattlesnake Crotalus durissus terrificus and with venoms from several snakes of the Bothrops genus was detected only at concentrations higher than 1 *g/ml sample. A conventional ELISA is also described for the detection of antibodies against TsV. Analysis by Western Blot (WB) demonstrated a 25-kDa protein band common to TsV and to the venoms of Bothrops moojeni, B. jararacussu and B. jararaca. Venom from C. d. terrificus exhibited WB cross-reactive bands of 16 and 25 kDa with TsV
Assuntos
Coelhos , Animais , Masculino , Imunização , Imunoglobulina G/análise , Venenos de Escorpião/imunologia , Western Blotting , Cromatografia de Afinidade , Reações Cruzadas , Venenos de Crotalídeos/imunologia , Ensaio de Imunoadsorção EnzimáticaRESUMO
Tityustoxin (TsTx), the purified venom of the Brazilian scorpion Tityus serrulatus, was inected intravenously (50 microng/Kg) into rats, producing a typical picture of chronic pancreatitis after 20 days. Nesidioblastosis, a lesions characterized by hyperplasia of the islets of Langerhans, was also detected in a high percentage (40%) of animals. TsTx-induced pancreatitis may be a useful model for the study of nesidioblastosis in laboratory animals
Assuntos
Ratos , Animais , Masculino , Neurotoxinas/toxicidade , Pancreatopatias/patologia , Pancreatite/induzido quimicamente , Venenos de Escorpião/toxicidade , Doença Crônica , Injeções Intravenosas , Pancreatite/patologia , Venenos de Escorpião/administração & dosagemRESUMO
Relata-se poliarterite nodosa na infancia associada a endocardite infecciosa descrita em seus aspectos clinicos laboratoriais e histopatologicos.Discutem-se as dificuldades de diagnostico, etiopatogenia, assim como o envolvimento neurologico central e a resposta a terapeutica instituida.com dois modelos e resistencia, sensive