Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Adicionar filtros








Intervalo de ano
1.
Rev. cuba. med. trop ; 52(1): 15-20, Jan.-Apr. 2000.
Artigo em Espanhol | LILACS | ID: lil-333503

RESUMO

For the detection of Enterovirus, we devised a direct economical method of polymerase chain reaction which does not require a previous extraction of ribonucleic acid and uses infected cell culture supernatants. The system was developed on the basis of universal primers of Enterovirus and specific primers of vaccinal strain Sabin 1. The achieved results proved that the non-existence of methods of RNA extraction and purification prior to the reaction does not affect the susceptibility and specificity of the system, in the rapid detection of Enterovirus genomes and identification of vaccinal strains of poliovirus.


Assuntos
Humanos , Enterovirus , Reação em Cadeia da Polimerase/métodos , Sequência de Bases , DNA Viral , Enterovirus , Infecções por Enterovirus/diagnóstico , Infecções por Enterovirus/virologia , Meningoencefalite , Dados de Sequência Molecular , RNA Viral , Sensibilidade e Especificidade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA