Response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by HPTLC method

Objective: To optimize the ultrasonication method for efficient extraction of P-sitosterol and lupeol from the roots of Astragalus atropilosus using Box-Behnken design of response surface methodology (RSM), and its validation by high performance thin layer chromatography (HPTLC) method. Methods: Ultrasonication method was used to extract β-sitosterol and lupeol from Astragalus atropilosus (roots). RSM was used to optimize the different extraction parameters viz. liquid to solid ratio (10-14 mL/g), temperature (60-80 °C) and time (40-60 min) to maximize the yield of β-sitosterol and lupeol. The quantitative estimation of β-sitosterol and lupeol was done in chloroform extract of Astragalus atropilosus by validated HPTLC method on 10 cm × 20 cm glass-backed silica gel 60F

Flavonoids from morus nigra L. grown in Saudi Arabia

The ethyl acetate extract of Morus nigra L. leaves grown in Saudi Arabia afforded three known flavonoids; kacnipferol, Kaempferol-3-O-beta-D-glucopyranoside [astragalin] and quercetin-3-O-beta-D-glucopyranoside [isoquercitrin]. The Structures were determined utilizing different physical, chemical and spectroscopic data

Triterpenoids from acacia tortilis

From the leaves of Acacia tortilis, a new triterpene of lupane series along, with four known triterpenes have been isolated. Their structures were elucidated by chemical and physical data [IR. 1H-NMR, 13C-NMR and Mass spectra]