Effects of parenteral vitamin E and selenium supplementation on immune status of dogs vaccinated with subunit and somatic antigens against taenia hydatigena

Thirty clinically healthy dogs were divided into five equal groups; Gs 1 and 2 were vaccinated with subunit and somatic antigens [Ags] respectively in combination with vitamin E and selenium [Vit E/Se] supplement, Gs 3 and 4 were vaccinated with subunit and somatic Ags respectively and group 5 was kept unvaccinated as control positive. Dogs in the vaccinated-Vit E/Se supplemented groups had a significantly greater [P

Comparative characterization and DNA analysis of hydatid and cysticercus taenuicollis cyst Fluids

Molecular technology is providing highly improved methods for identification of parasites, either of medical, veterinary, biological importance or interest. Hydatid fluid of sheep [HFS], camel [HFC], equine [HFE] and pig [HFP] origin and also Cysticercus taenuicollis fluid of sheep [CTFS] and pig [CTFP] origin were used in this investigation. Electrophoretic profile of metacestode fluid antigens using SDS-PAGE revealed multiple components in both high and low molecular weight ranges. There was extensive electrophoretic similarity between the investigated fluid antigens especially at 83 KDa and a common band for all antigens except CTFP at 243 KDa. Extensive cross-reactivity between metacestode fluid antigens in EITB using hyper-immune serum of hydatid cyst fluid of camel origin. There was common polypeptide band at 83 KDa recognized among all species. Cross-reactive components between all antigens except CTFS recognized by antiserum of HFC at 116 KDa and also between all antigens except CTFS recognized by antiserum of HFC at 116 KDa and also between all antigens except CTFS recognized by antiserum of HFC at 116 KDa and also between all antigens except HFS at 95 KDa. Further investigations will be necessary to isolate the, cross-reactive antigens and to evaluate their potency in protection against heterologous infection. The amplified restriction fragment length polymorphism [AFLP] technique is a relatively new method for the analysis of polymorphism that has not yet been widely used in parasitology. In this article, DNA analysis gave similar fragment for HFS, HFE, HFC, HFP, CTFS and CTFP at 293 Kpb and a common fragment at 305 Kpb in C. taenuicollis and hydatid fluids expect camel origin. These findings may have important consequences for human health and the control of hydatid and cysticercosis diseases