RESUMO
In this study, the pilot production of aerobic bioreactor tropical theileriosis vaccine was optimized with the aim of immunological assays for further mass production. We have shown earlier the delayed type hypersensitivity [DTH] assay could be used for evaluating the immunity and memory cells against specific Theileria antigen in vaccinated animals. In addition, TNF-alpha is the principle cytokine in modulating the cytotoxic activity of cytotoxic T-lymphocytes [CTL]. Immunological analysis of the vaccine was performed by using two cell mediated immunity [CMI] in vitro and in vivo DTH test [Theilerin] and TNF-alpha assay. The results of immune responses of susceptible immunized cattle by bioreactor vaccine in comparison with conventional flask vaccine revealed a significant stimulation of immune cells by transcription of high level of TNF-alpha and positive reaction against Theileria antigen in Theilerin skin test [DTH]. The equal immunological results achieved in both above mentioned vaccines verified the satisfactory immunity for aerobic bioreactor theileriosis vaccine for advance mass vaccination in the field on a large scale