Serum paraoxonase, arylesterase, and glutathione-s-transferase activities and oxidative stress levels in patients with mushroom poisoning

OBJECTIVES: Consumption of toxic species of mushrooms may have detrimental effects and increase oxidative stress. Paraoxonase, arylesterase and glutathione-S-transferase are antioxidants that resist oxidative stress. In this study, we analyzed the changes in these enzymes during intoxication due to mushrooms. METHODS: The study enrolled 49 adult patients with a diagnosis of mushroom poisoning according to clinical findings and 49 healthy volunteers as the control group. The patients with mild clinical findings were hospitalized due to the possibility that the patient had also eaten the mushrooms and due to clinical findings in the late period, which could be fatal. Paraoxonase, arylesterase, and glutathione-S-transferase concentrations, as well as total antioxidant and oxidant status, were determined in the 49 patients and 49 healthy volunteers by taking blood samples in the emergency department. RESULTS: While paraoxonase, arylesterase, and total antioxidant status were significantly decreased in the patient group (p<0.05), glutathione-S-transferase, total oxidant status and the oxidative stress index were significantly higher (p<0.05). There was a positive correlation between the hospitalization time and the oxidative stress index (r=0.752, p<0.001), whereas a negative correlation was found with glutathione-S-transferase (r=-0.420, p=0.003). CONCLUSION: We observed a significant decrease in paraoxonase and arylesterase and an increase in glutathione-S-transferase and oxidative stress indexes in patients with mushroom poisoning, indicating that these patients had an oxidative status. In particular, a low total antioxidant status and high oxidative stress index may gain importance in terms of the assessment of hospitalization duration.

Seroprevalence of Brucella in an elderly population in mid-Anatolia, Turkey.

Brucellosis is a widespread infectious disease in the mid-Anatolia regions of Turkey. This study investigated the prevalence of Brucella infection in elderly people of this region. In total, 750 elderly subjects were chosen by a simple random-sampling method. Blood samples were evaluated by the Brucella Wright agglutination test. Brucella seropositivity was detected in 24 (3.2%) of the 750 subjects. Although there was no statistical correlation between Brucella seropositivity and sex or educational level (p > 0.05), seropositivity was statistically significant with subjects exposed to risk factors (p < 0.001). It is concluded that Brucella infection is still an important public-health problem in the cities of mid-Anatolia.