[ immunohistochemical comparative evaluation of CK13 and CK18 expression in odontogenic cysts and ameloblastoma]

Evaluating cytokeratin contents of odontogenic cysts and tumors can be one of the important aspects in comparative study of these lesions. The aim of this study was to evaluate expression of CK13 and CK18 in odontogenic cysts and ameloblastomas. In this study, radicular cysts [20 cases], dentigerous cysts [20 cases], OKC [20 cases], unicystic ameloblastoma [20 cases] and solid ameloblastoma [20 cases] were evaluated. Then, 3-4 micro m sections were prepared and stained with immuhistochemical procedure [avidin- biotin method. The obtained data by microscopic consideration was analyzed with statistical tests such as: Chi-square, Mann-Whitney, Wilcoxon [p<0.05]. All of the odontogenic cysts and cystic parts of unicystic ameloblastoma expressed CK13 in the upper layers [middle and superficial] more than the deeper ones [basal and parabasal]. In ameloblastomas, CK 13 expression in stellate reticulum and acanthomatose component was more than preameloblasts. Expression of CK 18 in the upper layers [middle and superficial] was more than deeper layers [basal and parabasal] in radicular and dentigerous cysts. Results of immunoreactivity with CK18 in OKC was negative in 100% of cases. In addition, ameloblastomas [unicystic and solid] did have not significant differences in expression of CK13 and CK18. CK13 did not show any differences between these lesions but significant difference in CK18 expression at all 4 layers of the epithelium in odontogenic cysts and ameloblastomas may indicate the different nature of these lesions and probably confirms this suggestion that unicystic ameloblastoma is initiated as a neoplasm essentially, no from neoplastic change of an odontogenic cyst, in particular dentigerous cyst. The same CK 13 and CK18 expression pattern in unicystic and solid ameloblastomas reveals the same biomolecular nature despite different gross and clinical features

[Study of correlation of stress and geographic tongue lesion in patients referring to Isfahan Dentistry School]

Geographic tongue lesion or benign migratory glossitis is a lesion with prevalence of 1-3% that involves all age and sex groups. Etiology of this lesion is unknown, but some references have pointed out to relationship of stress and this lesion. The aim of this study is to introduce stress as one of the factors in geographic tongue incidence. This research was of a case-control type and our sample in case group included 30 patients affected with geographic tongue lesion which visited Isfahan Dental School department of oral medicine. These patients were randomly selected from men and women. Patients were explained about importance of this disease and diagnosis of it. Standard stress meter questionnaire of Klementz and Roberts was completed by patients and witness group and information was analyzed statistically by T-test. Witness groups were selected from fellows or family members of patients because of similarities in circumstances between two groups. Information gathered from questionnaire showed that average stress number in case group was 386 and in witness group 3032. T-test showed significant difference between stress number of two groups. Average age in case group was 32 years and witness group 42 years, and this difference was significant, But geographic tongue didn't have significant correlation with sex, educational degree, income, number of children, time spent on studying and place of residence. Nowadays, Psychosomatic diseases are very important. Based on the results of this research, it seems that stress is a significant factor in causing geographic tongue. Although, stress is not the only etiologic factor in causing geographic tongue, but its role as psychologic factor cannot be denied