[ effect of adult mouse lung extracts growth factors in differentiation of umbilical cord stem cells into blood cells in vitro condition]

Stem cells [SCs] have great therapeutic indication due to their potency of self-renewal, multilineage differentiation, feasibility and safety for donors. In this study, adult mouse lung extracts containing hematopoietic growth factors were administered to umbilical cord, and evaluated the differentiation of umbilical cord stem cells into erythroid and myeloid lineages. In this basic and practical research, SCs were isolated from umbilical cord by enzyme digestion and cultured in appropriate culture medium. Subjects were divided into four groups: Experimental groups 1 and 2 [E1 and E2] which were exposed to 50% and 70% concentration of lung extract for 7 days, respectively, sham [Sh] group which did not exposed to lung extract and cultured for 7 days, and control group [C]. E1, E2 and Sh groups were incubated for 7 days. All groups were evaluated by alkaline phosphatase detection kit for stem cells. Then, blood cells count and hematopoietic growth factors were assessed. ANOVA was used for data analysis. There were significant changes in E2 groups as compared with Sh and C groups, so that E2 group cells were differentiated into erythroid and myeloid lineages. Growth factors in lung extract could have stimulatory effects on umbilical cord stem cell differentiation into blood cells

[Protective effects of sodium tungestate on pancreas of diabetic male rats and its histopathological alteration]

Diabetes is a common disorder characterized by abnormal metabolism of carbohydrates, caused by insulin secretion deficiency and or insulin cell membrane receptors dysfunction. In this study, the effects of sodium tungstate on blood sugar and pancreatic islands were investigated in diabetic rats. Forty-eight male Wistar rats, each weighting 250 grams, were studied in eight groups of six. Group one was considered as control 1 and did not receive any drug. Groups 2 and 3 received sodium tungstate at dose of 1.75 and 2.25 g/lit for ad libitum for 60 days. Group 4, as control 2, was diabetized by single dose injection of 60 mg/kg streptozocin [STZ]. Groups 5 and 6 received 1.75 and 2.25 g/lit of sodium tungstate ad libitum for 15 days then were diabetized and received sodium tungstate for another 45 days in the same manner. Groups 7 and 8 were diabetized, and then were treated with sodium tungstate for 60 days. Blood levels of glucose and histopathologic findings of pancreas were evaluated at the end of study. Administration of sodium tungstate at dose of 1.75 and 2.25 g/lit, before and after diabetes, led to a significant blood sugar decrease. Sodium tungstate has had a positive, but not significant, effect on the number and diameters of Langerhans islands. This study showed that sodium tungstate decreases blood sugar, increases the number of cells in each island, regenerates Langerhans islands and prevents further development of diabetes

[Matricaria chamomilla extract infusion in to nucleus paragigantocellularis attenuates most of morphine withdrawal syndrome signs in rats]

Some reports show that co-administration of Matricaria chamomilla [MC] extract with morphine, greatly attenuate the development of morphine dependence and inhibit the expression of abstinence syndrome in morphine-dependent animals. Locus Coeruleus [LC] and nucleus paragigantocellularis [PGi] play an important role in developing symptoms of opiate withdrawal. The objective of this study was to determine the effects of Matricaria chamomilla extract infusion into PGi on morphine withdrawal syndrome signs [MWS] of rats. Thirty male rats [weight: 250-300gr] were surgically implanted with cannula at the PGi and then tested in 2groups: saline [control group] and morphine [twice daily for 7 days]. The dose of morphine on the first and second days was 2.5 mg/kg and was doubled every day. On 7[th] day, the animals received the last injection of morphine [50mg/kg] and divided in 4 subgroups: the morphine group [which only received morphine] and three MC groups [which received 1 micro l of MC extract with the concentrations of 10, 25, 50 micro g/micro l, 5 min before naloxone administration]. In the end of the training day [7[th] day] all groups were received naloxone [5mg/kg IP] 3h after last injection of morphine and then the frequencies of withdrawal behavior [jumping, climbing] were assessed for 30 minute. Our results showed that central administration of MC extract, especially at high doses [25 micro g/micro l], significantly attenuates most signs of the morphine withdrawal syndrome. These results suggest that the injection of MC extract into the PGi may be helpful for morphine withdrawal syndrome treatment

[Relationship between computer user involvement, computer attitude and computer anxiety in community health centers]

Use of computer has been increased during the recent years. There are numerous factors affecting computer use in different organizations, while user's involvement is among the most common influencing issues. The aim of the present study was to assess the relationship between computer user involvement and computer attitude and anxiety in community health centers. It was a descriptive study. Totally, 319 nurses and staff working in community health centers were included. Data were collected using a questionnaire composed of 3 parts: computer users' involvement, attitude towards the computer, and computer anxiety. Results showed that most of the respondents, 86.8%, were women and 11.6% were men. Most of the male candidates [42.9%] aged 45-54 years while most of the females [42.1%] aged 35-44 years. Users' involvement induce a positive attitude towards the computer [p<0.0001]. Indeed, users' involvement could increase positive attitude toward computer for patient care and patient confidentiality and can reduce computer anxiety [p<0.05]. Results have revealed that in the pretest analysis, most of the subjects had a moderate attitude and knowledge, however, following the education, most of the cases acquired suitable attitude and knowledge [p<0.001], while the differences among controls were not statistically significant. For providing successful implementation and development of computerized information systems, user's involvement is an important issue. Therefore, this factor should be considered by managers