Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Adenocarcinoma/complicações , Adenocarcinoma/patologia , Adenocarcinoma/epidemiologia , Neoplasias do Colo/complicações , Neoplasias do Colo/patologia , Neoplasias do Colo/epidemiologia , Período Pós-Operatório , Neoplasias Retais/complicações , Neoplasias Retais/patologia , Neoplasias Retais/epidemiologia , Fatores Etários , Distribuição por Sexo , Avaliação de Estado de Karnofsky , México/epidemiologiaRESUMO
Background: Several studies have reported that variants rs16969968 G>A of the CHRNA5 gene and CYP2A6*12 of the CYP2A6 gene are associated with smoking and smoking refusal, respectively. In addition, some studies report that a higher cigarette consumption is associated with low body mass index (BMI). Aim: To analyze the allele and genotypic frequencies of these variants and their impact on smoking and BMI. Material and Methods: A blood sample was obtained and a survey about smoking habits was answered by 319 university students aged 18 to 35 years (127 women, 171 smokers), living in Northeastern Mexico. Genetic variants were studied by polymerase chain reaction/restriction fragment length polymorphism and their frequencies were associated with smoking and BMI. Results: No associations were found between the analyzed variants and smoking in the study groups. However, there was an association among non-smoking subjects between the A allele of rs16969968 and high a BMI (p < 0.01). Conclusions: This last variant may be involved in food-addiction disorders.
Assuntos
Adolescente , Adulto , Feminino , Humanos , Masculino , Adulto Jovem , Índice de Massa Corporal , /genética , Frequência do Gene , Proteínas do Tecido Nervoso/genética , Receptores Nicotínicos/genética , Fumar/genética , Estudos Transversais , Variação Genética/genética , Genótipo , México , Nicotina/metabolismo , Reação em Cadeia da Polimerase/métodos , Polimorfismo Genético/genéticaRESUMO
The characteristics of tuberculosis (TB) patients related to a chain of recent TB transmissions were investigated. Mycobacterium tuberculosis (MTB) isolates (120) were genotyped using the restriction fragment length polymorphism-IS6110 (R), spacer oligotyping (S) and mycobacterial interspersed repetitive units-variable number of tandem repeats (M) methods. The MTB isolates were clustered and the clusters were grouped according to the similarities of their genotypes. Spearman’s rank correlation coefficients between the groups of MTB isolates with similar genotypes and those patient characteristics indicating a risk for a pulmonary TB (PTB) chain transmission were ana- lysed. The isolates showing similar genotypes were distributed as follows: SMR (5%), SM (12.5%), SR (1.67%), MR (0%), S (46.67%), M (5%) and R (0%). The remaining 35 cases were orphans. SMR exhibited a significant correlation (p < 0.05) with visits to clinics, municipalities and comorbidities (primarily diabetes mellitus). S correlated with drug consumption and M with comorbidities. SMR is needed to identify a social network in metropolitan areas for PTB transmission and S and M are able to detect risk factors as secondary components of a transmission chain of TB.