A quick and economical method to isolate plasmid DNA for large scale sequencing.

Isolating plasmid DNA for large scale sequencing demands automated liquid handling facility which is expensive and labs with limited resources cannot afford them. In view of the limitations of resources and time we have designed and standardized a simple method based on lysing the bacterial cells by boiling to isolate the plasmid DNA, which can be subsequently sequenced on automated DNA sequencer without the need for further purification. The sequencing quality and the number of base read through are comparable to the standard procedure followed for isolating plasmid DNA.