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China Journal of Chinese Materia Medica ; (24): 502-504, 2008.
Artigo em Inglês | WPRIM | ID: wpr-284458

RESUMO

<p><b>OBJECTIVE</b>This research focused on analyzing the differences of 5S rRNA gene spacer sequences on Swertia mussotii and its commonly used adulterants, including S. franchetiana, S. wolfangiana and S. chirayita.</p><p><b>METHOD</b>DNA was extracted from the collected Swertia samples. 5S rRNA intergenic spacers were amplified by PCR, sequenced and analyzed.</p><p><b>RESULT</b>5S rRNA gene spacer sequences were different between S. mussotii and its other three adulterants. Sequence divergence among species ranged from 30.6% to 65.0%.</p><p><b>CONCLUSION</b>5S rRNA spacers may be used as molecular authentication markers to differentiate S. mussotii and other commonly used Swertia adulterants. This result provides reliable and simple reference for the authentication of Swertia genus species.</p>


Assuntos
Sequência de Bases , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , RNA Ribossômico 5S , Genética , Homologia de Sequência do Ácido Nucleico , Swertia , Classificação , Genética
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