Bisphenol-A induces oxidative damage in the liver of chicken embryos

Oxidative stress mechanisms are involved in the embryotoxicity. The objective of this study was to assess hepatotoxicity of bisphenol-A [BPA] in chicken embryos. Fertile eggs were randomly divided into 4 groups; three experimental and one control groups, [N=15, for each group]. Embryos were administered 50, 100 and 200 PPM BPA, and incubated for 48 h at 37[degree]C with a relative humidity of 63%. The experiment was terminated on day 20 of incubation. Then, the embryos were decapitated and livers of embryos were collected for biochemical analysis. The total antioxidant capacity, alondialdehyde [MDA], glutathione [GSH], carotenoid and total protein levels were measured by spectrophotometer. The result of the present study indicated that the levels of total antioxidant in the livers of embryos exposed to 200 PPM BPA were higher than control and other groups, as well as the levels of MDA compared to control group [p<0.05]. The levels of GSH, total carotenoids and total protein were higher in all groups exposed to BPA than control group [p<0.05]. In addition, protein concentration in 200 PPM group was higher than of other groups [p<0.001]. So far, BPA may lead to induce toxic response of oxidative system in liver throughout embryonic period

cytoprotective effects of morus alba leaves in cultured fetus fibroblast cells against hyperglycemia

Diabetes is a chronic disease characterized by high blood glucose levels. Medicinal plants have useful source of hypoglycemic compounds and play important role in preventing the progress of this disease. Morus alba leaves is natural therapeutic agent shown hypoglycemic effects. In this study, the protective effects of alcoholic extract Morus alba leaves on fetus fibroblast cells under hyperglycemic condition were assessed. In this experimental study the cells were treated with six different concentrations of extracts. The nontoxic concentration of plant extract was measured with MTT assay in fetus fibroblast cells. Then, the fetus fibroblast cells were incubated with high dose of glucose and plant extract at concentrations of 1000 ppm. DNA synthesis, as marker of cell proliferation was determined. There were significant changes in cell proliferation in treated group with plant extract. Morus alba leaves could cause cell attachment and proliferation. The attachment of cells to culture plates has been shown to be affected by plant extract. The data obtained from this study approved that Morus alba leaves has cytoprotective effects against hyperglycemia

antioxidant and Flavonoids contents of Althaea officinalis L. flowers based on their color

There has been a growing interest in finding plants with biological active ingredients for medicinal application. Three colors of petals of Althaea officinalis [A. officinalis] flowers, i.e., pink, reddish pink, and white were examined for total antioxidant activity and flavonoids content. The reddish pink flowers of A. officinalis have more antioxidant activity and the power of antioxidant activity was reddish pink > pink > white. Findings suggest that the dark color can serve as an indicator of antioxidant content of the plant. Flavonoid content was highest in white flower thus this result indicated that flowers with light color can be considered for medicinal uses