Sliver nanoparticles accelerate skin wound healing in mice [Mus musculus] through suppression of innate immune system

This study aimed to find the effects of silver nanoparticles [Ag-NPs] [40 nm] on skin wound healing in mice Mus musculus when innate immune system has been suppressed. A group of 50 BALB/c mice of about 8 weeks [weighting 24.2 +/- 3.0 g] were randomly divided into two groups: Ag-NPs and control group, each with 25 mice. Once a day at the same time, a volume of 50 microliters from the nanosilver solution [10ppm] was applied to the wound bed in the Ag-NPs group while in the untreated [control] group no nanosilver solution was used but the wound area was washed by a physiological solution. The experiment lasted for 14. Transforming growth factor beta [TGF-beta], complement component C3, and two other immune system factors involving in inflammation, namely C-reactive protein [CRP] and rheumatoid factor [RF] in sera of both groups were assessed and then confirmed by complement CH50 level of the blood. The results show that wound healing is a complex process involving coordinated interactions between diverse immunological and biological systems and that Ag-NPs significantly accelerated wound healing and reduce scar appearance through suppression of immune system as indicated by decreasing levels of all inflammatory factors measured in this study. Exposure of mice to Ag-NPs can result in significant changes in innate immune function at the molecular levels. The study improves our understanding of nanoparticle interaction with components of the immune system and suggests that Ag-NPs have strong anti-inflammatory effects on skin wound healing and reduce scarring

Histopathological effects of nanosilver [Ag-NPs] in liver after dermal exposure during wound healing

With the advent of nanotechnology, significant progress has been made in the area of nanoscale materials such as nanosilver [Ag-Nps]. These nanoparticles have a wide range of applications and been used for antimicrobial purposes for more than a century. However, little attention has been paid to the toxicity of nanosilver wound dressing. This study was designed to investigate the possible histopathological toxicity of Ag-NPs in liver of mice during wound healing. A group of 50 female BALB/c mice of about 8 weeks were randomly divided into two groups: Ag-NPs and control groups [n=25]. After creating similar wound on the backs of all animals, the wound bed was treated in Ag-NPs group, with a volume of 50 microliters of the nanosilver solution [10ppm] ,and in control group, with the same amount of distilled water. The experiment lasted for 14 days. Histopathaological samplings of liver were conducted on days 2, 7 and 14 of the experiment. Histopathological studies demonstrated time-dependent changes in mice liver treated with Ag-NPs compared to control group. Some changes include dilation in central venous, hyperemia, cell swelling, increase of Kupffer and inflammatory cells. This study suggests that use of nanosilver for wound healing may cause a mild toxicity, as indicated by time-dependent toxic responses in liver tissue. However, this issue will have to be considered more extensively in further studies

Subacute dermal toxicity investigation of nanosilver on serum chemical biomarkers in male mice

Nanosilver is one of the most widely used nanomaterials due to its strong antimicrobial activity. Thus, because of increasing potential for exposure of human to nanosilver, there is an increasing concern about possible side effects of these nanoparticles. In this study, we tested the potential dermal toxicity of nanosilver bandage on serum chemical biomarkers in mice. In this study, 20 male BALB/c mice were randomly allocated into the treatment and control groups [n=10]. After general anesthesia and shaving the back of all animals in near the vertebral column, in the nanosilver group, a volume of 50microl of 10 microg/ml of nanosilver solution [40 nm], and in the control group the same amount of distilled water was added to the sterile bandage of mice, then the bandages were fixed on the skin surface with cloth glue. After 3 and 7 days, the bandages were opened and serum levels of blood urea nitrogen [BUN], creatinine [Cr], alanine aminotransferase [ALT] and aspartate aminotransferase [AST] were measured by using standard kits for two groups of mice. In treatment group, a significant increase in ALT, AST and BUN levels were observed compared with control group during experiment periods [p<0.05], but there wasn't a significant increase in Cr level in treatment group during experiment periods [p>0.05]. The present results indicated that the dermal absorption of 10 microg/ml nanosilver [40 nm] can lead to hepatotoxicity and renal toxicity in mice