RESUMO
Electrolyzed-reduced water (ERW) scavenges reactive oxygen species and is a powerful anti-oxidant. A positive correlation between oxidative stress and aging has been proved in many model organisms. In Caenorhabditis elegans, many long-lived mutants showed reduced fertility as a trade off against longevity phenotype. We aimed to study the effect of ERW on oxidative stress, fertility and lifespan of C. elegans. We also investigated the genetic pathway involved in the effect of ERW on resistance to oxidative stress and lifespan. We compared lifespan and fertility of worms in media prepared with distilled water and ERW. ERW significantly extended lifespan and increased the number of progeny produced. Then the effect of ERW on resistance to oxidative stress and lifespan of long-lived mutants was determined. ERW increased resistance to oxidative stress and lifespan of eat-2, a genetic model of dietary restriction, but had no effect on those of age-1, which is involved in insulin/insulin-like growth factor (IGF)-1-like signal. In addition, knockdown of daf-16, the downstream mediator of insulin/IGF-1-like signal, completely prevented the effect of ERW on lifespan. These findings suggest that ERW can extend lifespan without accompanying reduced fertility and modulate resistance to oxidative stress and lifespan via insulin/IGF-1-like signal in C. elegans.
Assuntos
Animais , Antioxidantes/química , Antioxidantes/farmacologia , Caenorhabditis elegans/efeitos dos fármacos , Longevidade/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Água/química , Envelhecimento/efeitos dos fármacos , Proteínas de Caenorhabditis elegans/genética , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Fertilidade/efeitos dos fármacos , Fertilidade/genética , Fator de Crescimento Insulin-Like I/metabolismo , Insulina/metabolismo , Longevidade/genética , Análise de Sobrevida , Transdução de Sinais/efeitos dos fármacos , Fatores de Transcrição/genéticaRESUMO
We tested the antibacterial properties of a mix of minerals consisting mainly of sericite, talc, and halloysite from Korea. The preparation showed clear growth inhibition of the gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, and the grampositive bacteria Staphylococcus aureus subsp. aureus, S. epidermidis, and Bacillus cereus, as well as the anaerobic bacterium Propionibacterium acnes. These results indicate that this preparation, made from ore minerals indigenous to Korea, could be used to develop new antibacterial reagents.