RESUMO
Abstract Pediococcus acidilactici strain S1, a lactic acid-fermenting bacterium, was isolated from makgeolli-a Korean traditional fermented alcoholic beverage. Here we report the 1,980,172 bp (G + C content, 42%) genome sequence of Pediococcus acidilactici strain S1 with 1,525 protein-coding sequences (CDS), of which 47% could be assigned to recognized functional genes. The genome sequence of the strain S1 might provide insights into the genetic basis of the lactic acid bacterium with alcohol-tolerant.
Assuntos
Genoma Bacteriano , Ácido Láctico/metabolismo , Bebidas Alcoólicas/microbiologia , Pediococcus acidilactici/isolamento & purificação , Pediococcus acidilactici/genética , Sequência de Bases , República da Coreia , Fermentação , Pediococcus acidilactici/metabolismo , Sequenciamento Completo do GenomaRESUMO
Abstract Pediococcus acidilactici strain K3 is an alcohol-tolerant lactic acid bacterium isolated from nuruk, which is a traditional Korean fermentation starter for makgeolli brewing. Draft genome of this strain was approximately 1,991,399 bp (G+C content, 42.1%) with 1525 protein-coding sequences (CDS), of which 44% were assigned to recognized functional genes. This draft genome sequence data of the strain K3 will provide insights into the genetic basis of its alcohol-tolerance.
Assuntos
Adaptação Biológica/efeitos dos fármacos , Adaptação Biológica/genética , Genoma Bacteriano , Etanol/farmacologia , Pediococcus acidilactici/efeitos dos fármacos , Pediococcus acidilactici/genética , Ácido Láctico/biossíntese , Biologia Computacional/métodos , Genômica/métodos , Etanol/metabolismo , Fermentação , Sequenciamento de Nucleotídeos em Larga Escala , Anotação de Sequência Molecular , Pediococcus acidilactici/isolamento & purificação , Pediococcus acidilactici/metabolismoRESUMO
Electrolyzed-reduced water (ERW) scavenges reactive oxygen species and is a powerful anti-oxidant. A positive correlation between oxidative stress and aging has been proved in many model organisms. In Caenorhabditis elegans, many long-lived mutants showed reduced fertility as a trade off against longevity phenotype. We aimed to study the effect of ERW on oxidative stress, fertility and lifespan of C. elegans. We also investigated the genetic pathway involved in the effect of ERW on resistance to oxidative stress and lifespan. We compared lifespan and fertility of worms in media prepared with distilled water and ERW. ERW significantly extended lifespan and increased the number of progeny produced. Then the effect of ERW on resistance to oxidative stress and lifespan of long-lived mutants was determined. ERW increased resistance to oxidative stress and lifespan of eat-2, a genetic model of dietary restriction, but had no effect on those of age-1, which is involved in insulin/insulin-like growth factor (IGF)-1-like signal. In addition, knockdown of daf-16, the downstream mediator of insulin/IGF-1-like signal, completely prevented the effect of ERW on lifespan. These findings suggest that ERW can extend lifespan without accompanying reduced fertility and modulate resistance to oxidative stress and lifespan via insulin/IGF-1-like signal in C. elegans.