RESUMO
Indisputable population exposure to widespread electromagnetic fields, has grown concerns over the probable health effects of these fields. The present study was aimed to examine the possible effects of 50 Hz extremely low frequency electromagnetic field [ELF-EMF] exposure on the number and quality of mice blastocysts. In present study, 66 NMRI pregnant females divided into two treated and non-treated groups. The treated group exposed to ELF-EMF [50 Hz and 6×10[-3] T]. Subsequently, embryos were collected by flushing the uterine horn and Fallopian tubes on the day 3 of gestation. Number of trophoectoderm [TE] and Inner Cell Mass [ICM] cells in blastocysts were determined after differential nuclei staining using a modified method. Furthermore, number of all flushed blastocysts calculated in each group. There was no significant difference in mean number of blastocysts in treated [6.64 +/- 1.34] and none treated [8.22 +/- 1.59] groups. In treated group, there were significant decreased in total cell number of blastocysts [p=0.000], number of ICM cells [p=0.000], and number of TE cells [p=0.001] whereas the ratio of ICM/TE cells increased [p=0.002]. The data indicate that ELF-EMF is able to affect cellular composition of blastocysts, but it can't omit total volume of blastocysts
RESUMO
Low electromagnetic fields [LEMF] are produced by instruments which are works with electricity. This study was done to determine the effect of LEMF on fetal death and bone marrow megakaryocytes in NMRI mouse neonates. In this experimental study 64 females' mice with 6-8 old weeks were used. 2 female mice coupled with one male, and positive vaginal plaque was interpreted as the zero day of pregnancy [GD=0], The pregnant mice were randomly categorized into control and experimental groups. The experimental group were exposed to 50HZ, 0.5 mT Low electromagnetic fields on 7-11 days of pregnant period [8h/d]. The weight of neonate and death fetus were studied after delivery. The live neonates were dissected on 15[th] day, and 1 ml of bone marrow was extracted from Tibia and vertebral column, by pressing method. The bone marrow cells suspended in 1:1 IMDM in 15cc [FULCON] tubule and cells was counted with neobar lam. The data were tested by t-student test; significance was set up at p<0.05. There was significant differences between the mean weight of one day neonate in cases with controls [P<0.05]. The mean of dead fetus in experimental group was higher than controls [P<0.05]. The mean of megakaryocytes numbers higher than controls, but this differences was not significant. This study showed that the number of megakaryocytes and fetal death were increased by low electromagnetic fields exposure during pregnancy