RESUMO
Introduction: Cancer chemotherapy induced diarrhea(CCID) is a frequent problem during anticancer treatment.Patho-physiology of CCID differs between chemotherapeuticagents. Neither there is specific treatment for CCID noradequate experimental models. The objective of this study wasto develop a relevant experimental animal model of CCID,which can help in development of specific therapy for CCID.Material and methods: In this study thirty six albino rats ofeither sex were divided into 6 groups of 6 animals each. GroupI rats received distilled water and served as control. GroupII,III,IV, V, VI received 5-Fluorouracil(5-FU) once intraperitoneal(IP) at doses 10,20,30,40 and 50 mg/kg respectivelyand served as test groups for experimental model of CID.Standard parameters like stool samples for quality& quantity,incidence of diarrhea, histopathology of intestine and fataloutcome in rats were recorded.Results: Out of test groups, rats treated with 5-FU 30mg/kg IP single dose developed diarrhea in 50% rats,mean timefor onset of diarrhea was 84±16.7 hours and duration was62±9.16 hours, diarrhea subsided within 7 days with mildchanges of intestinal histopathology and 16.7% mortality.Occurrence of diarrhea, change in intestinal histopathologywas not remarkable with dosage less than 30mg/kg. In 5 FU40 mg/kg & 50mg/kg group incidence of diarrhea increased to66.6% but death rate were further increased to 33.3% in5 FU40 mg/kg& 83.3% with 50mg/kg.Conclusion: The results suggest 5-FU 30mg/kg single doseIP to albino rats can be used as a suitable experimental modelfor CCID for evaluation of novel potential candidate drugs fortreatment of CCID.
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Human ocular infestation by live filarial worm is a rare occurrence and has been reported mostly form South-East Asia. It involves the eyelids, conjunctiva, cornea, anterior chamber and uvea. No case of intravitreal Brugian microfilaria / adult worm has been found by Medline search. Here we report a case of live intravitreal adult Brugian filaria, where the parasite was successfully removed by pars plana vitrectomy. Identification of the worm was done by light microscopy and confirmed by immuno chromatographic test.