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Indian J Exp Biol ; 2000 Jun; 38(6): 617-20
Artigo em Inglês | IMSEAR | ID: sea-63463

RESUMO

High frequency of streptomycin resistant variants of Lycopersicon esculentum were isolated on selective shoot regeneration medium supplemented with IAA (0.5 mg/L), zeatin (1.5 mg/L) and streptomycin sulphate (500 mg/L). Nonmutagenized (controls) and NMU treated cotyledons were placed on shoot regeneration medium supplemented with antibiotic streptomycin. Resistant shoots appeared at a high frequency in mutagenized cotyledons, whereas in controls morphogenesis was suppressed, accompanied by bleaching. Shoot regeneration occurred from the nodular tissues developed at the cut ends of cotyledons. Resistant shoots developed into complete plantlets on rooting medium containing selective concentration of antibiotic. Stability of streptomycin resistance was confirmed by leaf assay and reciprocal crosses between streptomycin-resistant and sensitive plants.


Assuntos
Cruzamento , Cruzamentos Genéticos , Meios de Cultura , Resistência a Medicamentos/genética , Ácidos Indolacéticos/farmacologia , Solanum lycopersicum/efeitos dos fármacos , Metilnitrosoureia/farmacologia , Morfogênese/efeitos dos fármacos , Mutagênese , Mutagênicos/farmacologia , Técnicas de Cultura de Órgãos , Brotos de Planta/efeitos dos fármacos , Plastídeos/efeitos dos fármacos , RNA de Plantas/antagonistas & inibidores , RNA Ribossômico/antagonistas & inibidores , Sementes/efeitos dos fármacos , Seleção Genética , Estreptomicina/farmacologia , Zeatina/farmacologia
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