Adult type anomalous left coronary artery from pulmonary artery.

Anomalous origin of Left Coronary Artery from Pulmonary Artery (ALCAPA) is a rare congenital anomaly. Its survival into adulthood is further rare. Clinical manifestations result from evolving morphological - functional alterations in pulmonary circulation that occur after the birth. We report a case of 43 year old adult patient with effort angina and without any ECG or Echo abnormalities. On coronary angiography, typical anatomy of ALCAPA was revealed.

Effect of inducers on hepatic microsomal mixed function oxidase system of male rats during food restriction.

Phenobarbital (PB; 80 mg/kg, ip) or 3-methylcholantrene (3-MC; 20 mg/kg, ip) was administered to Wistar male rats at the end of the feeding period of 30 days and the effects of food restriction (FR) and FR followed by inducer treatment on hepatic drug metabolizing enzymes, microsomal electron transport components, NADPH dependent lipid peroxidation and glutathione-s-transferase activities were studied. In both, PB and 3-MC treatment, the magnitude of increase in microsomal protein content, cytochrome b5 and aminopyrine N-demethylase (APND) activity was less in FR animals than in ad libitum fed; while cytochrome P-450 levels and activities of cytochrome c reductase and acetanilide hydroxylase (ACOH) were higher in FR animals. NADPH dependent lipid peroxidation and cytosolic glutathione-s-transferase activity were also enhanced due to PB and 3-MC treatment but the magnitude of increase was less in FR animals. The ACOH activity increased to a greater extent than APND activity in FR animals following PB and 3-MC treatment. It is suggested that the response to inducers in the FR animals differ from that in the ad libitum fed rats.

Mixed function oxidase system in hepatic and extrahepatic tissues of developing chickens following various treatments.

Alterations in hepatic and extrahepatic protein content, activities of drug enzymes, lipid peroxidation and hydrogen peroxide formation and levels of microsomal electron transport components were examined in developing chickens during treatment with phenobarbital (PB), benzene and 1,1,1,-trichloro 2,2, bis (p-chlorophenyl) ethane (DDT) treated group of 4-8 weeks old chickens. Activities of hepatic and extrahepatic (kidney, lung, intestine) tissues enzymes were induced during PB treatment in all groups of chicken. However benzene and DDT treated group of chickens recorded decrease in activities of drug enzymes. Magnitude of increase due to PB administration was much more in liver as compared to kidney, lung and intestine. Hydrogen peroxide and lipid peroxide formation significantly increased in all tissues during treatments of benzene and DDT. Levels of cyto P-450, cyto b5 and cyto c-reductase, lipid peroxidation and hydrogen peroxide formation increased in all groups of chickens during various treatments. The results suggest that chicken liver contains more drug enzyme activities and electron transport components during development as compared to other tissues. Benzene and DDT administration resulted into decrease in the activities of drug enzymes.

Purification of erythrocyte cytochrome P-450 from goat and chick.

Cytochrome P-450 has been purified from goat and chick erythrocytes and characterized. Goat erythrocyte cytochrome P-450 content was higher than that of chick erythrocytes cytochrome P-450. Elution profile of purified protein from DEAE-cellulose column showed a single peak. The catalytic activities of aminopyrine-N-demethylase and acetanilide hydroxylase were found to be higher in purified proteins. Molecular weight was determined by SDS-polyacrylamide gel electrophoresis.

Hepatic mixed function oxidase system and effect of phenobarbital, 3-methylcholanthrene and 1, 1, 1-trichloro-2, 2-bis(p-chlorophenyl)ethane in developing chickens.

Contents of hepatic microsomal protein, aminopyrine N-demethylase, acetanilide hydroxylase, aniline hydroxylase, hydrogen peroxide formation, cytochrome-c-reductase, cytochrome b5 and cytochrome P-450 were examined in control, phenobarbital (PB), 3-methylcholanthrene (3-MC) and 1, 1, 1-trichloro-2, 2-bis(p-chlorophenyl)ethane (DDT) treated group of 1-28 days old chickens. Increase in aminopyrine N-demethylase, acetanilide hydroxylase, aniline hydroxylase, cytochrome-c-reductase, cytochrome b5 and cytochrome P-450 was noticed at all stages of development during administration of PB and 3-MC. But these enzyme activities were not always paralleled by increase in age. Aminopyrine N-demethylase was increased in early stages only during DDT administration, which indicates that the form of cytochrome P-450, responsible for aminopyrine N-demethylation is present in early stages only. However, acetanilide hydroxylase was decreased in all stages of development, in postnatal development the basal activities of the enzymes for various substrates do not exhibit identical pattern, the degree of inducibility by inducers varied in relation to age of animal. Hydrogen peroxide formation increased in all stages of developing chickens due to the administration of PB and DDT. It however decreased due to 3-MC administration which may be due to induction of high spin cytochrome P-450.