Linkage map construction for silkworm [Bombyx mori L] based on amplified fragment length polymorphism markers

The domesticated silkworm, Bombyx mori, is of high commercial importance as a silk producer and is also widely used for implementation of basic and applied research. It is important to understand its genome organization using molecular markers for genetic studies and for breeding purposes. In this study, a genetic linkage map using 204 amplified fragment length polymorphism [AFLP] markers was developed. Twenty PstI/TaqI primer combinations were used to genotype 78 progenies from an F2 population of the P107_Khorasan Lemon cross. Each primer combination generated an average of 10.2 AFLP markers qualified for linkage mapping. All the 204 AFLP markers were assigned to 12 linkage groups at the Logarithm of Odds [LOD] threshold of 2. The number of markers in the linkage groups ranged from 2 to 53. There were seven major linkage groups with 13-53 markers and five small linkage groups with 2-6 markers. The 12 linkage groups varied in length from 12.3 to 938.4 cM and the total length of linkage map was 4262 cM, giving an average marker resolution of 20.89 cM. This study presents the preliminary step for further marker-assisted research on silkworm, including Quantitative Trait Loci [QTL] and introgression analyses

Study of genetic variation in wild diploid wheat [triticum boeoticum] from Iran using AFLP markers

Little information is available regarding genetic variation in wild wheat relatives from Iran. In this study, genetic diversity of 36 populations of wild einkorn wheat, Triticum boeoticum, was studied using amplified fragment length polymorphism [AFLP] primer combinations. Seventeen AFLP primer combinations led to amplify 979 scorable fragments ranging from 50 to 500 bp and of these, 429 [44%] were polymorphic across the 36 populations. The average Dice genetic similarity between T. boeoticum populations was 0.67 [range= 0.18-0.98]. The dendrogram derived by unweighted pair group method with arithmetic mean algorithm [UPGMA] analysis revealed three main groups. PCO analysis also confirmed subgrouping obtained by cluster analysis. The measured relative genetic distances among accessions was not correlated with geographical distances of places of their origins, indicating that the populations are genetically different. The results demonstrated that AFLP technology is a suitable technique for genetic resource management in T. boeoticum populations of these studied origin sites