Serum Metabonomics Analysis of Colitis Associated Cancer Mice Intervened by Hedyotis diffusa Based on UPLC-Q-TOF-MS / 中国实验方剂学杂志

Objective:To investigate the intervention of <italic>Hedyotis diffusa</italic> （HDW） on colitis associated cancer （CAC） model mice and explore its mechanism. Method:The CAC mouse model was established by synergistic action of azoxymethane （AOM） and dextran sulfate sodium （DSS）. The intervention of HDW on CAC mice was evaluated by disease activity index （DAI）， colonic tissue morphology， pathological injury score and tumorigenesis rate. Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS） and multivariate statistical analysis were used to analyze the metabonomics of mice serum and to explore the mechanism of HDW intervention on CAC. Result:HDW could significantly improve the general condition of CAC mice， decrease DAI， colon gross morphological score， histopathological score and tumorigenesis rate. Compared with the normal group， 38 kinds of differential metabolites were screened in the model group， including 11 potential biomarkers， involving 11 main metabolic pathways. HDW could significantly regulate 9 kinds of differential metabolites ［niacinamide， uridine， 4-pyridoxic acid， LysoPC （18∶0）， LysoPE （0∶0/20∶0）， myo-inositol， purine， sphinganine 1-phosphate and tetradecanedioic acid］ in the model group， including 2 kinds of potential biomarkers （myo-inositol and niacinamide）， and HDW could regulate nicotinate and nicotinamide metabolism and inositol phosphate metabolism. Conclusion:HDW has a therapeutic effect on CAC， which may be achieved by regulation of energy metabolism and glucose metabolism.

Comparison of two reconstruction methods of alimentary canal after total gastrectomy / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To evaluate the clinical value of two alimentary reconstruction methods after total gastrectomy for gastric cancer.</p><p><b>METHODS</b>Thirty- one patients undergoing total gastrectomy for gastric cancer were prospectively randomly divided into two groups: single-canal jejunum interposition (n=16, group A) and P pouch with Roux-en-Y esophagojejunostomy (n=15, group B). All patients were followed up for one year. The serum nutritional parameters,body weight change and clinical effects between the two groups were compared.</p><p><b>RESULTS</b>Both of the reconstruction types had the replacement of gastric function. The hemoglobin level was (102+/- 11)g/L, the total protein level (6.8+/- 3.9)g/L, and the body weight loss (1.0+/- 1.7)kg in group A, while (98+/- 12)g/L, (6.3+/- 3.0)g/L and (4.0+/- 2.3)kg in group B. There were significant differences between the two groups (all P< 0.05).</p><p><b>CONCLUSION</b>The single-canal jejunum interposition reconstruction after total gastrectomy (Henley) is a better surgical method.</p>