ffects of Zuogui Pills on kidney-yin deficiency syndrome of premature ovarian insufficiency rats / 国际中医中药杂志

Objective:To study the effects of Zuogui Pills on rats with kidney-yin deficiency syndrome of premature ovarian insufficiency.Methods:Totally 40 SD female unmated rats were randomly divided into blank group, model group, Zuogui Pills group and Bujiale group, with 10 rats in each group. Except for blank group, rats in other groups were subcutaneously injected with ZP3 and gavaged with levothyroxine sodium to induce kidney-yin deficiency syndrome model of premature ovarian insufficiency. At the same time of modeling, Zuogui Pills group and Bujiale group received corresponding drugs for gavage, and the other groups received corresponding solvent for gavage, once a day, for consecutive 21 days. On day 0, 7, 14 and 21, ear temperature and body weight of rats were measured, and the ovarian index, uterus index and thyroid index were calculated. Serum levels of adenosine cyclic phosphate (cAMP), cyclic guanosine phosphate (cGMP), Cortisol, follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E 2) were detected by ELISA. Pathological changes of ovary was observed with HE staining. Results:On day 14 and 21, compared with model group, the body weight of rats in Zuogui Pills group increased ( P<0.05), and the ear temperature decreased ( P<0.05); compared with model group, the ovarian index, uterine index and thyroid index of rats in Zuogui Pills group decreased ( P<0.05), the levels of serum cAMP/cGMP, cortisol, FSH and LH decreased ( P<0.05), and the level of E 2 increased ( P<0.05). Conclusion:Zuogui Pills have certain improvement effect on rats with kidney-yin deficiency syndrome induced by levothyroxine sodium tablets combined with ZP3.

Construction and application of mCherry red fluorescent protein fusion expression system in lactic acid bacteria / 生物工程学报

In order to enrich the library of domestic research about new red fluorescent marker in lactic acid bacteria (LAB), we described a new fusion expression system in Lactobacillus plantarum WCFS1 based on the pSIP vector. This system contained red fluorescent protein mCherry as a marker and bile salt hydrolase gene (bsh) as a reporter gene. Moreover, in this study, four different promoters (PsppA, PldhL, P32 and PslpA) were used to regulate the expression of the fusion protein mCherry-BSH, completing the inducible and constitutive expression in lactic acid bacteria. The recombinant protein mCherry-BSH presented activity of red fluorescence and bile salt hydrolase (BSH). The successful construction of the fusion expression system in LAB using a red fluorescent protein mCherry provides favorable conditions for the distribution, intestinal colonization and survival rate of lactic acid bacteria, so as to reveal the function mechanism of its probiotic characteristics; and the system also could lay the foundation for researches on protein expression, cellular localization and properties identification of active protein in lactic acid bacteria.

Professor WANG Peijuan's Experience in Treating Polycystic Ovary Syndrome / 浙江中医药大学学报

[Objective]Investigating Professor WANG Peijuan's clinical experience in treating PCOS from studying the kidney. [Method] This paper mainly studied by arranging and researching the tutor's theory and experience about treating PCOS that based on the practice in clinic with the teacher as a self perception,the collection about the PCOS cases of tutor's clinical treatment and the analysis of national and international relative literature combined with self-awareness. [Result]Professor WANG thinks that PCOS is the disorder of qi and blood and the transformation failure of kidney-Tiangui-Chongren-uterus, which leads to phlegm and blood stasis, blocking in the uterus and making menstruation lose its inhere periodicity. The disease is of the kidney deficiency and phlegm stagnation syndrome.[Conclusion]Professor WANG holds that to use the method of tonifying kidney properly and attach importance to the three categories of etiologic factors will be effective to the treatment of PCOS. Her experience can provide the basis for diagnosis and treatment about the disease.

The Establishment of Autoimmune Premature Ovarian Failure (POF) Model Using B6AF1 Female Mice / 世界科学技术-中医药现代化

This study aimed at exploring the modeling and identification method of autoimmune POF by the ZP3 polypeptide with the provision of scientific basis for the prevention and treatment of POF.B6AF1 female mice,the hybridization of A/J male mice and C57BL/6 female mice,was immunized by zona pellucida polypeptide fragments.Then,the mice estrous cycle was detected by vaginal exfoliated cells Pasteur's staining.After the 14-day modeling,the mice model was identified on the 7th and 14th day,respectively.Ovarian tissue morphology and anti-zona pellucida antibodies immunofluorescence change were detected by H&E staining and ZP3 immunofluorescence staining.Serum hormone levels of E2 and FSH in the mice were tested by ELISA,with the combination of estrous cycle,ovarian tissue morphology and anti-zona pellucida antibodies immunofluorescence change to judge the success of the POF mice model.It was found that ZP3 polypeptide immunized mice appeared the disorder of estrous cycle after 14 days,so did the marked inflammation observed by ovarian biopsy,a clear transparent zone by ZP3 immunofluorescence staining,and the decrease of serum E2 declined and the increase of serum FSH by ELISA.However,no significant change was found on the 7th day in the modeling.In conclusion,ZP3 polypeptide induced autoimmune POF mice model was established successfully,featuring high incidence and simple method and laying a foundation for the further study of autoimmune POF.

Analysis of Treatment Ideology of Premature Ovarian Failure through Fu Qingzhu's Differentiation and Treatment of “Menstruation Stopped When Not Old Yet” / 浙江中医药大学学报

Purpose] The article analyzed the rules of diagnosis and treatment of “menstruation stopped when not old yet”in the Fuqingzhunvke Section · Menstruation Chapter, which provides a new thought of diagnosing and treating for the treatment of premature ovarian failure. [Methods] Because of the characteristics of the provisions in the Fuqingzhunvke is consistent with the clinical symptoms of premature ovarian failure in the modern medicine. The article analyzed the cause, pathogenesis, principle-method-recipe-medicine and treatment to explore the treatment of premature ovarian failure. [Result] The characteristics of the provisions in the Fu Qingzhu Nvke are consistent with the clinical symptoms of premature ovarian failure in the modern medicine. Fushi thought that premature ovarian failure derived from heart, liver, spleen and kidney, treatment should reinforce kidney water;puts forward some new ideas for qi depression, nourishing liver to resolve depression method;pay attention to tonifying spleen, nourishing heart and tranquilizing mind; reminding relieving depression lies in strengthening the body resistance. [Conclusion] Through analyzing the etiology, pathogenesis, treatment of premature ovarian failure, to clarify the etiology, pathogenesis, treatment of premature ovarian failure. The study of classics of traditional Chinese medicine provides a new method for the treatment of premature ovarian failure. Making the past serve the present, the clinical curative effect will be improved.

Theoretical Discussion of Phlegmy Wet and Polycystic Ovary Syndrome / 浙江中医药大学学报

Objective] Investigate the relationship between polycystic ovary syndrome and phlegm wet, lay a theoretical foundation for from the wet treatment. [Method] According to the diagnostic standard of PCOS, try from the signs and symptoms, laboratory indexes, imaging manifestations of three aspects, analyzes and demonstrates the relationship between the disease with phlegm wet. From curing phlegm wet as the breakthrough point, dialectical analysis and tailored medication,explore the treatment effect and advantage. [Result] PCOS shown by a series of symptoms and signs are associated with phlegm wet blocking uterus; Insulin resistance, lipid metabolic disorder, leptin, and tumor necrosis factor pathways are closely associated with phlegm wet; Polycystic ovary is visual performance of phlegmy wet block uterus.Arguments from phlegmy wet, and guiding drugs, combined with the spirit, exercise, diet therapy have a significant clinical curative effect .[Conclusion] In this paper, starting from the theory of phlegm wet treatment of the disease,curative effect is distinct and has high practical value. Also it demonstrates the scientific and particularity of Chinese medicine on disease. It will provide a new train of thought for the gynecologic diseases and the other diseases in the future.

Research the Idea of Aromatherapy as Adjuvant Therapy for Postpartum Depression / 浙江中医药大学学报

Purpose]Providing theoretical basis for using aromatherapy as an adjuvant therapy to treat postpartum depression. [Method]The research summarizes the etiological factors, pathogenesis of postpartum depression and il ustrates the basis of treatment for aromatherapy in the perspectives of traditional Chinese medicine and western medicine. [Result]It confirms the theoretical basis for using aromatherapy as adjuvant therapy to treat postpartum depression. [Conclusion] As an adjuvant therapy, aromatherapy could effectively al eviate the suffering of patients and it would have a bright future.

Determination of peoniflorin,ferulic acid and icariin in Bushen Huoxue Granule / 中成药

AIM: To establish the method of determining peoniflorin,ferulic acid and icariin in Bushen Huoxue Granule(Herba Epimedii,Radix Angelicae Sinensis,Radix Paeoniae Alba,etc.).METHODS: HPLC-DAD was used to determine peoniflorin,ferulic acid and icariin.The samples were separated on Alltima C18(5 ?m,250 mm ? 4.6 mm) with mobile phase consisted of methanol-0.1% phosphoric acid(gradient elution).RESULTS: The average recoveries of peoniflorin,ferulic acid and icariin were 96.7%,97.5%,99.6% and RSD were 1.37%,3.13%,0.96%.respectively.The peoniflorin,ferulic acid and icariin samples showed a good relationship in the ranges of 0.318-3.970 ?g,0.096-1.200 ?g,0.414-5.175 ?g in the chosen HPLC condition.CONCLUSION: The method is simple,accurate and reproducible and can be used for the quality control of granule.