RESUMO
<p><b>OBJECTIVE</b>To screen genes related to peritoneal metastasis of colorectal cancer.</p><p><b>METHODS</b>Specimens of primary cancer and normal mucosa tissues were collected from 3 patients with peritoneal metastasis of colorectal cancer. The total RNA were extracted and inversely transcribed into cDNA to synthesize aRNA using in vitro RNA synthesis. The synthesized aRNA, after labeling with Cy3, were hybridized with the whole human genome oligo microarray. The Empirical Bayes method was used to screen the differentially expressed genes, followed by confirmation of the selected genes by semi-quantitative RT-PCR.</p><p><b>RESULTS</b>With a threshold of P≤0.05, a total of 105 differentially expressed genes were identified in primary cancer lesions, including 42 up-regulated and 63 down-regulated genes. Three of the up-regulated genes (S100P, PRDX1 and SLPI) were selected and confirmed by RT-PCR, which yielded results consistent with those from gene microarray.</p><p><b>CONCLUSION</b>Gene microarray technique can provide valuable clues for locating the tumor markers of peritoneal metastasis in colorectal cancer patients.</p>
Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adenocarcinoma , Genética , Adenocarcinoma Mucinoso , Genética , Proteínas de Ligação ao Cálcio , Genética , Metabolismo , Neoplasias Colorretais , Genética , Patologia , Perfilação da Expressão Gênica , Estudo de Associação Genômica Ampla , Proteínas de Neoplasias , Genética , Metabolismo , Análise de Sequência com Séries de Oligonucleotídeos , Métodos , Neoplasias Peritoneais , Genética , Peroxirredoxinas , Genética , Metabolismo , Inibidor Secretado de Peptidases Leucocitárias , Genética , MetabolismoRESUMO
<p><b>OBJECTIVE</b>To explore the effect of low-energy semiconductor laser intranasal irradiation of the blood on blood coagulation status in healthy pregnant women at term.</p><p><b>METHODS</b>Low-energy semiconductor laser was introduced into the nasal cavity in 126 healthy pregnant women at term and 123 healthy young unmarried women as the control group. The plasma prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), and fibrinogen levels were examined using transmissive turbidimetry after the therapy.</p><p><b>RESULTS</b>PT, APTT and TT levels were significantly lowered, whereas fibrinogen level significantly increased in the healthy pregnant women before the laser therapy as compared with those in the control group (P<0.01). After intranasal laser therapy, these parameters were significantly improved in the healthy pregnant women (P<0.05) although there were differences from those of the control group.</p><p><b>CONCLUSION</b>Low-energy semiconductor laser intranasal irradiation of the blood can effectively improve high blood coagulation status in healthy pregnant women at term.</p>