Efficacy of Shenqi Fuzheng Injection combined with chemotherapy in treatment of acute myeloid leukemia and its effect on body's immune function / 中草药

Objective: To observe the clinical efficacy of Shenqi Fuzheng Injection (SFI) combined with chemotherapy in the treatment of acute myeloid leukemia (AML) and the effect on body's immune function, and to explore the function and mechanism of adjuvant chemotherapy with SFI. Methods: One hundred and twelve patients were divided into two groups: the treatment group (n = 54) treated with DA protocol plus SFI and the control group (n = 58) treated with DA protocol alone. We observed the remission rate, mortality, the time of myelosuppression, the changes of T lymphocyte subsets; and the side-effects of chemotherapy for the patients in the two groups. Results: Comparing the remission rate and mortality rate of the patients between the two groups, there was no statistically significant difference (P > 0.05). The side-effects of the patients in the treatment group were more significantly reduced than those of the patients in the control group (P +/CD4+ cells, and higher cell rates of CD4+/CD8+ (P < 0.05). Conclusion: The SFI could shorten the time of myelosuppression induced by the chemotherapy in the treatment of AML, regulate the level of T lymphocyte subsets, enhance the active immune function of body, and reduce the relative side-effects of chemotherapy.

Diagnosis model of idiopathic thrombocytopenic purpura based on platelet differential proteome / 中国实验血液学杂志

This study was purposed to establish a new quick and simple diagnostic method with high sensitivity and good specificity for idiopathic thrombocytopenic purpura (ITP) and to evaluate its significance. 240 platelet lysates (from patients with ITP, leukemia, MDS, and healthy adults, each of 60 cases) were randomly assigned to training set (120 cases) or validation set (120 cases), all of them were detected by surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS), in order to identify the differentially expressed protein, the diagnostic model was established by means of artificial neural network (ANN), and was validated by blind test with SPSS 17.0. The results showed that 5 marked proteins significantly differentially expressed (P < 0.01), m/z of highly expressed proteins were 2234.30, 3476.36, and 7526.29, m/z of low expressed proteins were 4990.02 and 5152.39, respectively. The sensitivity and specificity of diagnostic model were 80.6% and 77.3% respectively. The area under the ROC curve consisting of the output value of artificial neura1 network was 0.837. Efficacy of the model was validated by means of blinded test. It is concluded that the ANN model is useful for clinical diagnosis of ITP on the basis of platelet protein fingerprint spectrum.

Study on the serum level of granulocyte-macrophage colony stimulating factor in some hematopathy patients / 中国实验血液学杂志

In order to explore the serum level of granulocyte-macrophage colony stimulating factor (GM-CSF) in hematopathy patients, radioimmunoassay was used to detect GM-CSF level in serum from 163 cases of hematopathy, including 36 chronic aplastic anemia, 42 chronic granulocytic leukemia, 54 acute myeloid leukemia, 31 acute lymphocytic leukemia, and 40 healthy adults as control. The results showed that the serum GM-CSF level increased in chronic aplastic anemia patients, and significantly decreased in acute and chronic leukemia patients. In conclusion, these findings indicated that secreting level of GM-CSF is abnormal in patients with acute/chronic leukemias and chronic aplastic anemia.