Separation and identification of glycoprotein in human serum of Fragile X syndrome

Background: Fragile X Syndrome (FXS) is the most common cause of inherited mental retardation. The absence of Fragile X Mental Retardation (FMRP) in Fragile X syndrome changes other proteins. Objective: To detect changes of glycoprotein in human serum of Fragile X syndrome. Subject and methods: Affinity chromatography with lectin concanavalin A (ConA) used to receive glycoprotein. The collected glycoprotein was then separated using 2-D electrophoresis. The protein spots were further excised, trypsin digested, and analyzed by nano LC couple with ESI-MS/MS and identified by MASCOT v1.8 software. Results and conclusion: 5 glycoproteins showed the different expression levels in the serum of Fragile X syndrome. Haptoglobin, Ig-J were increased and ceruloplasmin, transferring, Ig kappa were decreased. Using affinity chromatography with lectin concanavalin A (ConA), glycoprotein was received and divided on 2 ways electrokinetic chromatography. The mixture protein was identified with a reliability of 99.5% by 2 ways liquid chromatography combined with continuous spectrum mass.

The mortality during the 24 hours after admission at Nghe An Pediatric Hospital

Background: Pediatric emergency at the inter-hospital transfer lines is a serious problem in the care and treatment of children. 24 hours mortality after admission rose higher than previously.\r\n', u'Objectives: The aim of the study was to determine the 24 hours mortality and to analyze the causes of 24 hours mortality.\r\n', u'Subjects and methods: The study was carried out on 189 patients, who died during 24 hours after admission at Nghe An Pediatric hospital from July 2002 to June 2004.\r\n', u'Results: The results showed that the 24 hour mortality consisted of 77% of all deaths in the hospital. The neonatal mortality rate among the admitted patients was the highest. The most common causes of deaths were: respiratory and neurological diseases with 48% of the total (24% each) followed by injury (15%). Asphyxia/premature ranked third (9%). Surgical diseases consisted of 8.5% and infectious diseases contributed 6% of all deaths. \r\n', u'Conclusion: The interventions at hospital should prioritize the improvement of knowledge and skills on neurological, respiratory, trauma and surgical emergency care. The urgent need is also to train staff and to supply equipment for neonatal care. \r\n', u'