RESUMO
OBJECTIVE@#To evaluate the power of Identifiler System for paternity testing.@*METHODS@#A total of 3 277 paternity testing cases were studied using Identifiler System. The exclusion power and mutation rates of the Identifiler System were analysed in the paternity testing.@*RESULTS@#The cumulated power of exclusion was 0.999 998 827, and the cumulated discriminating power was 0.999 999 999 999 999 98, respectively. Of the 3 277 cases, paternity was confirmed in 2 863, but excluded in 347. Among this paternity testing, mutations involving a single STR locus were observed in 65 cases, while mutations involving 2 STR loci were observed in 2 cases.@*CONCLUSION@#The Identifiler System is powerful and reliable for paternity testing.
Assuntos
Humanos , Alelos , China , Impressões Digitais de DNA/métodos , Genética Forense/métodos , Testes Genéticos/métodos , Genética Populacional , Repetições de Microssatélites , Mutação , Paternidade , Reação em Cadeia da Polimerase/métodos , Probabilidade , Sequências de Repetição em Tandem/genéticaRESUMO
OBJECTIVE@#The aim was to investigate the polymorphisms of D6S1043 and D12S391 loci among Han population and evaluate their values in paternity testing. MERTHODS: By using fluorescence dye-labeled primers and capillary electrophoresis, the allele frequencies of the two STR loci among 192 unrelated individuals were investigated.@*RESULTS@#Twelve alleles were observed in both D6S1043 and D12S391 loci. The ranges of allele frequencies were from 0.0026 to 0.1719 and from 0.0026 to 0.2292, respectively. The discrimination power of D6S1043 and D12S391 were 0.9656 and 0.9510. The Average exclusion probability in paternity testing for duos were 0.573 and 0.510. The Average exclusion probability in paternity testing for trios were 0.731 and 0.679, respectively. The genotypes frequencies met Hardy-Weinberg equilibrium expectation.@*CONCLUSION@#The results show that D6S1043 and D12S391 have high values in forensic paternity testing.
Assuntos
Humanos , Alelos , China/etnologia , Impressões Digitais de DNA/métodos , Eletroforese Capilar , Medicina Legal/métodos , Frequência do Gene , Genética Populacional , Paternidade , Probabilidade , Sequências de Repetição em Tandem/genéticaRESUMO
OBJECTIVE@#Research of the hair damage due to perming, combing and stretching can be of important value for forensic hair individual identification.@*METHODS@#The normal human hairs were treated with perming combing and stretching, and the keratins of the damage hair were analysed by using SDS-PAGGE and laser densimeter.@*RESULTS@#Perming, combing and stretching brought about hair damage; The keratins of the damage hair were obviously reduced at the rang of molecular weight of 67,000-43,000 dalton.@*CONCLUSION@#The loss of the damage hair keratins were increased with the degree of the hair damage.