A new disubstituted succinate from the roots of Tagetes erecta / 药学学报

Nine compounds were isolated from 95% ethanol extract of the roots of Tagates erecta by silica gel column chromatography and Sephadex LH-20 chromatography. Their structures were identified by spectroscopic data as 5-hydroxymethylfurfuryl methyl succinate (1), 5,7,3'-trihydroxyl-3,6,4'-trimethoxylflavone (2), syringic acid (3), 5,7,4'-trihydroxyl-3,6-trimethoxylflavone (4), patuletin-4'-methoxyl-7-O-β-D-glucopyranoside (5), patulitrin (6), 5,3'-dihydroxyl-3,6,4'-trimethoxylflavone-7-O-β-D-glucopyranoside (7), (2,2'-biththiophen)-5-ol (8), and 3-hydroxyl-4-methoxyl benzoic acid (9). Among them, compound 1 is a new disubstituted succinate and compound 8 was isolated from a natural resource for the first time. Compounds 2, 4, 5 and 9 were isolated from this genus for the first time. By measuring the biological activity and virulence of different compounds against soybean cyst nematode, it has found that compounds 1-8 exhibited a toxic effect on soybean cyst nematode, and ED50 values indicate that compounds 3 and 7 are the most potent, with ED50 values of 0.008 μg·mL-1.

Research Process of Chemical Constituents and Pharmacological Effects of Tibetan Medicine Triphala / 世界科学技术-中医药现代化

Triphala consists of Terminalia chebula Retz, Terminalia bellirica (Gaertn.) Roxb. and Phyllanthus emblica L, which are made into Dasanguo Powder. Triphala is rich in a variety of chemical components, including tannins, phenolic acids, triterpenoids and flavonoids. The content of tannin is abundant in Triphala, which are often used as the main indicators of analysis. Modern research found that Triphala has a variety of pharmacological activities such as prevention of gastrointestinal diseases, antibacterial, anti-inflammatory, anti-oxidation, anti-tumor and so on. This paper briefly summarized the chemical constituents and pharmacological effects of Triphala, combining the relevant national and international literature in recent years to provide reference for the development and further study of Triphala.

Comprehensive Evaluation of the Quality of Tibetan Medicine Triphala's Tannin Parts Based on There Characteristic Components and the Total Tannin Content / 世界科学技术-中医药现代化

To determine there characteristic components content of Tibetan Medicine Triphala through establishing a HPLC method and its total tannin content through spectrophotometry. The chromatographic column of Agilent ZORBAX SB-Aq (250 × 4.6 mm, 5 μm) with methanol-0.1％ formic acid/water as the mobile phase, the flow rate 1 mL/min, and the detection wavelength 270 nm was applied to determine the Gallic acid, colijing and ellagic acid content in medicinal materials and the tannin parts of Tibetan Medicine Triphala. With the gallic acid as control group, total tannin content of Triphala and its tannin parts was determined through spectrophotometry. It revealed in the HPLC test that the linear range of gallic acid, coracine and ellagic acid was 0.91-4.55 μg, 0.274-1.368 μg and 0.329-2.634 μg respectively. It also showed that the average recovery rates of the three components in the medicinal materials were 101.06％, 101.72％and 100.27％ respectively. And the average recovery rates of the three components in the tannins were 100.4％, 100.85％and 101.70％ respectively. The result of spectrophotometry showed that gallic acid was linear in1.008-10.08 μg·mL-1, and that the recovery rate of medicinal materials and tannin parts were 100.25％ and 100.52％ respectively. The method is rapid, accurate and repeatable, and it can provide basis for the quality control of Tribescens and its tannins.

Study on Water-Soluble Constituents from Tibetan Medicine Terminalia billirica (Gaert.) Roxb / 世界科学技术-中医药现代化

This paper aimed at investigating the water-soluble constituents of Terminalia billirica (Gaert.) Roxb.Compounds were isolated and purified by Diaion HP-20, Sephadex LH-20, Toyopearl HW-40, MCI gel CHP 20 P and ODS column chromatographies. The chemical structures were elucidated according to spectral data and physicochemical properties. The results showed that ten compounds were isolated and identified as corilagin, salicoside, methyl-α-Darabinofuranoside, 1, 4-dimethyl-β-d-fructopyranose, β-fructopyranose, β-fructofuranose gallic acid, ellagic acid, 1, 2, 3, 4, 6-penta-O-galloyl-β-D-glucopyranonside, Chebulagic acid. Except compound 7 and 8, others compounds are separated from Terminalia billirica (Gaert.) Roxb for the first time, and except corilgin, the other compounds are separated from Terminalia for the first time.

Study on Rapid Identification of Constituents of Tibetan Medicine Terminalia chebular Retz. by HPLC/LTQ-Orbitrap MSn / 世界科学技术-中医药现代化

The aim of this work was to identify and analyze the chemical constituents of Terminalia chebular Retz by establishing high performance liquid chromatography-linear ion trap/electrostatic field orbitrap combined high resolution mass spectrometry (HPLC-LTQ-Orbitrap MS) technique. With the application of C18 reverse phase column (4.6 mm×250 mm, 5 μm), gradient elution was performed with methanol and water (0.2％ acetic acid) as mobile phase.In negative ionization mode, the data of LTQ-Orbitrap was collected. Accurate molecular mass of molecular ion peaks and fragment ions provided by the high-resolution mass spectrometry were compared with the literature and the reference substance to determine the possible structure of the compound. The results indicated that 62 compounds from the extract of Terminalia chebular Retz. were identified, including acids and hydrolysable tannins (60), triterpenoids (2) . There were14 compounds were detected from this specie for the first time. It is proved as an effective method to provide reference for Chemical composition mass spectrometry and the quality control in further phytochemical studies of Terminalia chebular Retz.

Study on Quality Evaluation of Terminalia Chebular Based on Simultaneous Determination of Total Tannin and 7 Components / 世界科学技术-中医药现代化

The spectrophotometric method was established for the determination of total tannin content in gardenia medicinal materials and tannins, and HPLC method was applied to simultaneously determine gallic acid, punical glucoside A, methyl gallate, punny glucoside B, corilagin, pentagalloyl glucose and the content of ellagic acid. Taking gallic acid as a reference substance, a phosphomolybdate tungstic acid colorimetric method (Pharmacopoeia method) was used to determine the total tannin content. With the chromatographic column Agilent ZORBAX SB-Aq-C18 (250 × 4.6 mm, 5 microns), mobile phase for phosphoric acid (0.1％), methanol, water flow rate of 1 ml/min, column temperature 30℃, detection wavelength of 270 nm, gradient elution, 7 kinds of component contents in myrobalan medicinal materials were measured at the same time. The result showed that the average content of total tannins of Radix Scutellariae was 30.31％, RSD was 0.55％; the average recovery rate was 99.70％, and the RSD was 21.97％ (n = 6) . In the scorpion medicinal materials, gallic acid, punical glucoside A, punny glucoside B, methyl gallate, punny glucoside B, creatin, pentagalloglucose and ellagic acid have a good linear relationship in their respective ranges. The recovery rate of the sample is between100.10％ and 102.77％. The establishment of the determination method of total tannin, gallic acid, punical glucoside, methyl gallate, corridain, pentagalloglucose and ellagic acid in the scorpion medicinal materials is simple and accurate, which has strong specificity and can be used for quality control of Terminalia chebular.

Study on HPLC Fingerprint of Terminalia chebular Retz Based on Chemometrics Analysis / 世界科学技术-中医药现代化

This paper was aimed to establish the HPLC fingerprint of Terminalia chebular Retz The analysis was performed on Agilent ZORBAX SB-Aq C18 column (4.6×250 mm, 5 μm) . The mobile phase was 0.1％ phosphate watermethanol solution with gradient elution. The flow rate was 1 mL·min-1. The temperature was maintained at 35℃. The detection wavelength was 270 nm. The injection volume was 20 μL. The chromatographic data of 10 samples was analyzed by similarity of chromatographic data, SPSS software and SIMCA software. The results showed that there were17 common peaks in the diagram. The similarity of 10 samples was between 0.984 and 0.999, which showed that there was no significant difference among these chemical components. The samples were classified into four types according to cluster analysis, which shared the similar results as the principal components analysis (PCA) and partial least sq uares analysis (PLS-DA) . VIP analysis result showed that peak 5 (methyl gallate), 11 and 1 (gallic acid), 10, 12 may be the main chromatographic peaks to identify the samples. It was concluded that chemometrics analysis could be used to analyze, find and distinguish the marker ingredients of Terminalia chebular Retz. This method was accurate and stable, which can provide a reference for the quality control and evaluation of Terminalia chebular Retz.

Extraction and Separation of Boron in Anhydrite and Gypsum Minerals and Its Isotopic Measurement by Thermal Ionization Mass Spectrometry / 分析化学

The anhydrite and gypsum are the main sulfate minerals during evaporation of seawater or lake.They record the information about relative hydrogeology and the composition of mother liquor.Boron is diffluent element, and often occurs in all kinds of evaporites.Presently, the boron isotope has been applied widely in mineral deposits forming, geochemistry and palaeoenvironment.However, there is little research about characteristic of boron isotope in anhydrite and gypsum minerals, because of the low content of boron and micro-solubility in water and hydrochloric acid.This study developed a method of extracting and purifying boron in anhydrite and gypsum by phase transformation and ion-exchange.Firstly, the samples were mixed with ammonium hydrogen carbonate to transform the calcium sulfate to calcium carbonate.And diluted hydrochloric acid (1 mol/L) was added to resolve calcium carbonate.The percent conversion was about 85%in the first stage, and up to complete resolution by repeating this process.Secondly, boron specific ion-exchange resin ( Amberlite IRA 743 ) was used to gather the boron ions fully and further refined the samples with more than 1 μg of boron by anionic and cationic resin mixed by Ion Exchange Ⅱ and Dowex 50 W × 8.Finally, according to the modified method by He, the values of boron isotope were determined by TIMS.The boron content is analytically pure gypsum was 3.501 ± 0.128 μg/g ( n=12 , RSD=3.6%) and the average recovery was 100.47%.Besides, the δ11B value of analytically pure gypsum added with NIST SRM 951 was 17.98‰±0.21‰ (n=3, RSD=1.2%).This method has good repeatability and can meet the requirements of boron isotopic measurement of anhydrite and gypsum.

Clinical Study on Modified Huanglian Wendan Decoction for Treatment of Tic Disorder Children with Syndrome of Wind Stirring Due to Phlegm-heat / 中国中医药信息杂志

Objective To observe the clinical efficacy of modified Huanglian Wendan Decoction for treatment of tic disorder (TD) children with syndrome of wind stirring due to phlegm-heat; To detect its effects on peripheral-blood miR-429 expression. Methods Eighty cases of TD children were randomly divided into treatment group and control group,with 40 cases in each group.In the treatment group,children were treated with modified Huanglian Wendan Decoction, one dosage a day, twice a day, orally. In the control group, TD children were treated with haloperidol, 0.03 mg/(kg?d), orally, according to the symptom to adjust dose, and the maximum amount did not exceed 0.08 mg/(kg?d). 30 d was a treatment course for both groups. The treatment lasted for three courses. Before and after 3 months of clinical observation, the severity of disease was evaluated by TCM syndrome and Yale Global Tic Severity Scale (YGTSS) score. Clinical efficacy and TCM syndrome efficacy were evaluated; the level of miR-429 in peripheral blood was detected; the adverse reactions were observed. Results The total effective rate of clinical efficacy was 82.5% (33/40) in treatment group and 80.0% (32/40) in the control group, without statistical significance (P>0.05); The total effective rate of TCM syndrome efficacy was 92.5% (37/40) in treatment group and 75.0% (30/40) in the control group, with statistical significance (P<0.05). Compared with before treatment, the YGTSS score and TCM syndrome score significantly decreased after treatment (P<0.01), and the TCM syndrome score in the treatment group was much lower than the control group after treatment (P<0.05). Compared with before treatment, miR-429 level in both groups increased obviously after treatment (P<0.01), and the miR-429 level in the treatment group was much higher than the control group after treatment (P<0.05). There were mild constipation (1 cases), diarrhea (2 cases), and anorexia (2 cases) in the treatment group, and obvious lethargy (3 cases) and dizziness (3 cases) in the control group. Conclusion Modified Huanglian Wendan Decoction shows obvious effects for treating TD, and can up-regulate miR-429 of peripheral-blood.

X-ray diagnosis and significance of degenerative osteoarthritis / 中国生化药物杂志

Objective To analyze the X-ray diagnosis and significance of degenerative joint disease. Methods From September 2015 to January 2017 in our hospital 86 cases of degenerative joint disease patients as the research object, a retrospective analysis of clinical data of the patients, in the premise of pathological diagnosis, all patients underwent X-ray diagnosis. Analysis of degenerative joint disease to X-ray diagnosis. Results Men showed left and right tibiofemoral joint and patellofemoral joint involvement; women showed left and right tibiofemoral joint and patellofemoral joint involvement. Patients showed more changes to the knee joint degenerative bone, periosteal thickening, stenosis, visible osteophytes, or bone hyperplasia, calcification of symptoms. 41 cases (47.6%) accompanied by joint swelling, 22 cases (25.6% limbs) joint involvement, 13 cases (15.1%) of spinal involvement, 10 cases (11.7%) with small joint involvement. Conclusion The X-ray diagnosis of degenerative joint disease, high clinical value, worthy of promotion.

Analysis of the effect of amiodarone combined with psychological intervention on arrhythmia and its effects on serum high sensitive C reactive protein / 中国生化药物杂志

Objective To investigate the clinical effect of amiodarone combined with psychological intervention on the treatment of the patients with arrhythmia and its influence on the serum high sensitive C reactive protein. Methods The control group were treated with amiodarone on the basis of routine treatment, the study group was given amiodarone combined with psychological intervention on the basis of routine treatment. The effect in the two groups before and after treatment and the hs-CRP level changes in the two groups were recorded. Results The total efficiency in the study group was 89.13%, which was higher than 71.74% in the control group(P<0.05). There was no significant difference in the level of hs-CRP between the two groups before treatment. After treatment, the hs-CRP in study group was better than that in control group (P<0.05). Conclusion On the basis of routine treatment, amiodarone combined with psychological intervention can significantly improve the clinical efficacy of arrhythmia patients, and significantly reduce the level of hs-CRP.

Clinical effect of clopidogrel in treatment of acute angina pectoris / 中国生化药物杂志

Objective To observe the clinical effect of acute angina pectoris in cardiovascular surgery. Methods 92 patients with acute angina were enrolled in our hospital from March 20 to March 2017 were randomly divided into study group and control group,with 46 cases in each group. The control group was treated with enalapril and aspirin enteric-coated tablets. The study group was treated with clopidogrel on the basis of the control group.The clinical effect of the two groups after treatment was observed, and the incidence, duration and total effective rate of acute angina were compared between the two groups. Results The incidence of acute angina pectoris and pain time in the study group were significantly lower than in the control group (P<0.05). The total effective rate of the treatment group was significantly higher than that of the control group (P<0.05). Conclusion The combination of enalapril, aspirin enteric-coated tablets and clopidogrel in patients with acute angina pectoris can effectively reduce the number of acute angina pectoris and shorten the duration of onset of pain. The clinical effect is significant and high clinical application value.

Clinical Observation ofChanhouFujiu Decoction plus Acupuncture-moxibustion in Preventing and Treating Incomplete Drug Abortion / 上海针灸杂志

Objective To observe the clinical efficacy ofChanhou Fujiu decoction plus acupuncture-moxibustion in preventing and treating incomplete drug abortion.Method A total of 204 patients in early-stage pregnancy asking for drug abortion were randomized into group A, group B, and group C, 68 cases in each group. Group A was given oral administration of Mifeprostone and Misoprostol tablets, plus Azithromycin dispersible tablet for prevention of infection. Group B was intervened by orally takingChanhou Fujiu decoction in addition to the treatment given to group A. Group C was intervened by acupuncture-moxibustion therapy based on the treatment given to group B. The vaginal bleeding time and amount, and the recovery of menstruation in the three groups were observed after the intervention, and the clinical efficacies were compared.Result Compared with group A, the vaginal bleeding amount after drug abortion was significantly smaller (P<0.05), the bleeding duration were significantly shorter (P<0.05), and time taken for the recovery of menstruation was markedly shorter (P<0.05) in group B and C, and there were no significant abnormal conditions in the menstrual periods (P>0.05). Compared with group B, group C had significantly smaller amount of vaginal bleeding (P<0.05) and shorter duration of vaginal bleeding (P<0.05), and there were no significant abnormal conditions in the time taken for the recovery of menstruation and menstrual periods (P>0.05). The total effective rate was 75.0％ in group B and 95.6％ in group C, both significantly higher than 58.8％ in group A (P<0.05), and the total effective rate in group C was markedly higher than that in group B (P<0.05). Conclusion Chanhou Fuyuan decoction plus acupuncture-moxibustion can effectively prevent and treat complications of drug abortion, and is an effective method in preventing and treating incomplete drug abortion.

Therapeutic Observation of Abdominal Acupuncture plus Chinese Medication for Ureteral Calculus / 上海针灸杂志

Objective To observe the clinical efficacy of abdominal acupuncture plus Chinese medication in treating ureteral calculus. MethodA total of 106 patients with ureteral calculus were randomized into an abdominal acupuncture group (53 cases) and a medication group (53 cases). The abdominal acupuncture group was intervened by abdominal acupuncture plus medication, with Shuifen (CV9), Xiawan (CV10), Jianli (CV11), Zhongji (CV3), Guanyuan (CV4) and Qihai (CV6) selected as the major points. The removal of stones and the release of pain were observed in the two groups, and the efficacies were compared between the two groups.Result The two treatment methods both effectively improved the pain and promoted the removal of stones; the Visual Analogue Scale (VAS) scores were significantly changed after the intervention in both groups (P<0.05). The time taken to mitigate and release pain was significantly shorter in the abdominal acupuncture group compared to the other group (P<0.01). The total effective rate was 88.7% (47/53) in the abdominal acupuncture group, superior to 73.6% (39/53) in the medication group (P<0.05).ConclusionAbdominal acupuncture plus medication can effectively mitigate pain, promote the removal of stones and reduce patient's sufferings in treating ureteral calculus.

X-ray diagnosis and significance of degenerative osteoarthritis / 中国生化药物杂志

Objective To analyze the X-ray diagnosis and significance of degenerative joint disease. Methods From September 2015 to January 2017 in our hospital 86 cases of degenerative joint disease patients as the research object, a retrospective analysis of clinical data of the patients, in the premise of pathological diagnosis, all patients underwent X-ray diagnosis. Analysis of degenerative joint disease to X-ray diagnosis. Results Men showed left and right tibiofemoral joint and patellofemoral joint involvement; women showed left and right tibiofemoral joint and patellofemoral joint involvement. Patients showed more changes to the knee joint degenerative bone, periosteal thickening, stenosis, visible osteophytes, or bone hyperplasia, calcification of symptoms. 41 cases (47.6%) accompanied by joint swelling, 22 cases (25.6% limbs) joint involvement, 13 cases (15.1%) of spinal involvement, 10 cases (11.7%) with small joint involvement. Conclusion The X-ray diagnosis of degenerative joint disease, high clinical value, worthy of promotion.

Analysis of the effect of amiodarone combined with psychological intervention on arrhythmia and its effects on serum high sensitive C reactive protein / 中国生化药物杂志

Objective To investigate the clinical effect of amiodarone combined with psychological intervention on the treatment of the patients with arrhythmia and its influence on the serum high sensitive C reactive protein. Methods The control group were treated with amiodarone on the basis of routine treatment, the study group was given amiodarone combined with psychological intervention on the basis of routine treatment. The effect in the two groups before and after treatment and the hs-CRP level changes in the two groups were recorded. Results The total efficiency in the study group was 89.13%, which was higher than 71.74% in the control group(P<0.05). There was no significant difference in the level of hs-CRP between the two groups before treatment. After treatment, the hs-CRP in study group was better than that in control group (P<0.05). Conclusion On the basis of routine treatment, amiodarone combined with psychological intervention can significantly improve the clinical efficacy of arrhythmia patients, and significantly reduce the level of hs-CRP.

Expression of Wntless in gastric carcinoma and its clinical significance / 基础医学与临床

Objective To explore the expression of Wnt secret protein Wntless in gastric carcinoma and normal mu-cosa,and to analyze the clinical significance of Wntless expression. Methods To detecte the expression of Wntless protein in gastric adenocarcinoma and normal gastric mucosa by immunohistochemistry. Results In 104 specimens of gastric cancer,the expression of Wntless in gastric adenocarcinoma was: 7.7% (8/104) cases were 0 or 1+, 36.5% (38/104) cases were 2+,and 55.8% (58/104) cases were 3+. While in normal mucosa,the expression of Wntless was:71.2% (74/104) cases were 0 or 1+, 19.2% (20/104) cases were 2+, and 9.6% (10/104) cases were 3+.The expression of Wntless in gastric adenocarcinoma was significantly higher than that in normal gas-tric mucosa (P<0.001). The expression of Wntless protein was positively correlated with tumor differentiation(P<0.05),and was positively correlated with lymph node metastasis (P<0.05).Conclusions Wntless is highly ex-pressed in gastric carcinomas and may have role in the oncogenesis. Wntless may be used as a new marker for pro-gression and metastasis of gastric carcinoma.

Clinical effect of clopidogrel in treatment of acute angina pectoris / 中国生化药物杂志

Objective To observe the clinical effect of acute angina pectoris in cardiovascular surgery. Methods 92 patients with acute angina were enrolled in our hospital from March 20 to March 2017 were randomly divided into study group and control group,with 46 cases in each group. The control group was treated with enalapril and aspirin enteric-coated tablets. The study group was treated with clopidogrel on the basis of the control group.The clinical effect of the two groups after treatment was observed, and the incidence, duration and total effective rate of acute angina were compared between the two groups. Results The incidence of acute angina pectoris and pain time in the study group were significantly lower than in the control group (P<0.05). The total effective rate of the treatment group was significantly higher than that of the control group (P<0.05). Conclusion The combination of enalapril, aspirin enteric-coated tablets and clopidogrel in patients with acute angina pectoris can effectively reduce the number of acute angina pectoris and shorten the duration of onset of pain. The clinical effect is significant and high clinical application value.

Clinical Features, Surgical Treatment and Prognosis in Acute Myocardial Infarction Patients With Ventricular Septal Rupture Complicating Cardiogenic Shock / 中国循环杂志

Objective:To explore the clinical features,surgical treatment and prognosis in acute myocardial infarction (AMI) patients with ventricular septal rupture (VSR) complicating cardiogenic shock (CS).Methods:A total of 77 AMI-VSR patients received surgical repair in our hospital from 2005-01 to 2015-05 were retrospectively studied.The patients were divided into 2 groups:CS group n=52 and Without CS group,n=25.Clinical features were compared between 2 groups and the outcomes in CS group were analyzed.Results:Both groups showed multiple aneurysm (73.0％ vs 68.0％);CS was more occurred in patients with posterior VSR (48.0％ vs 24.0％),P=0.044.Compared to Without CS group,CS group had the larger diameter of VSR (16.16±6.73) mm vs (11.86±4.62) mm,P=0.003;lower LVEF (45.0±8.8) ％ vs (47.9±12.3) ％,higher pre-operative application rates of IABP (34.6％ vs 0％) and vasoactive drugs (96.2％ vs 28.0％),more patients received emergent surgery (42.3％ vs 8.0％) and less patients received elective surgery (57.7％ vs 92.0％),all P＜0.05.In CS group,there were 3 in-hospital death,49 patients survived with the mean follow-up time at (4.5±3.1) years and 2 patients died during that period.Conclusion:Larger or posterior VSR were more likely to develop CS,the patients survived after surgical treatment may have good mid-term outcomes.

Acute toxicity investigation of recombinant trivalent human papillomavirus vaccine / 药物评价研究

objective To evaluate the acute toxicity of recombinant trivalent human papillomavirus (HPV) vaccines.Methods Wistar rats were randomly divided into negative control and HPV groups with 20 rats in each group.Rats in HPV groups were sc administered with a single-dose of 1.5 mL of HPV vaccines (three times of human dose/each rat),while rats in negative control group were given equal volume of saline.The clinical state of animals was observed,and the body mass changes were detected.Rats were dissected after 15 d of treatment and examined by gross pathology.Results Following a single injection with high-dose HPV vaccines,there were not obvious abnormalities in clinical symptom.Compared with negative control group,the body weight of rats from HPV vaccines-treated group had no significant difference.No obvious macro-pathological change was found in all animals.Conclusion Single sc injection with recombinant trivalent HPV vaccines is well tolerated and no obvious toxicological change is found in Wistar rats.These results will facilitate further preclinical safety studies of HPV vaccines.