RESUMO
The purpose of this in vitro study was to evaluate the antimicrobial effect of photodynamic therapy on Streptococcus mutans (ATCC 25175) suspensions, using a red laser for one minute in combination with toluidine blue O (TBO) or methylene blue (MB). Both photosensitizers were used in three concentrations (25, 10 and 5 mg/L). The activity of photosensitizers and laser irradiation were tested separately on the bacteria, as well as the irradiation of this light source in the presence of the TBO or MB. These groups were compared to a control group, in which the microorganism did not receive any treatment. The activity of both TBO and MB or laser irradiation, alone, were not able to reduce the number of S.mutans. In the groups of lethal photosensitization, a bacterial reduction of 70% for TBO and 73% for MB was observed when these photosensitizers were used at 25 mg/L and a reduction of 48% was observed for MB at 5mg/L. In other concentrations there were no significant differences in comparison to the control group. Both the TBO and the MB at 25 mg/L associated with a red laser had an excellent potential for use in PDT in lethal sensitization of S. mutans.
O objetivo deste estudo in vitro foi avaliar o efeito antimicrobiano da terapia fotodinamica em suspensoes de Streptococcus mutans (ATCC 25175), utilizando um laser vermelho durante um minuto associado a dois agentes fotossensibilizantes: azul de toluidina (TBO) ou azul de metileno (MB). Os agentes fotossensibilizantes foram utilizados em tres diferentes concentracoes (25, 10 and 5 mg/L). A atividade destes agentes e da fonte de luz foi testada separadamente sobre a suspensao bacteriana, assim como a irradiacao desta fonte de luz na presenca de TBO ou MB (terapia fotodinamica). Estes grupos foram comparados a um grupo controle, onde nenhum tratamento foi realizado. A aplicacao dos dois fotossensibilizantes (TBO ou MB) e da fonte de luz, separadamente, nao foi capaz de reduzir o numero de colonias viaveis do S. mutans. Nos grupos onde a terapia fotodinamica foi aplicada, uma reducao bacteriana de 70% foi observada para o TBO e de 73% para o MB, quando estes agentes foram utilizados na concentracao de 25 mg/L. O uso do MB a 5mg/L causou uma reducao de 48%. Para as outras concentracoes testadas nao se observou nenhuma reducao em relacao ao grupo controle. Pode-se concluir que tanto o TBO quanto o MB a 25 mg/L associados ao laser vermelho demonstraram um excelente potencial para promover a fotossensibilizacao letal do S. mutans.
RESUMO
OBJECTIVES: The objective of the present study was to analyze the superficial roughness and the interface between enamel and composite resin restorations after dental bleaching procedure. MATERIALS AND METHODS: Black's class V cavities were made and restored with composite resin, and the whole set, enamel-restorative material, was treated with 35% hydrogen peroxide. Seven procedures of 30 min each were performed. A profilometric assessment was carried out before and after the treatment of each sample, and roughness scores were obtained. Treated and untreated samples were analyzed under scanning electronic microscope and images of their surface were obtained. RESULTS AND CONCLUSION: The treatment with 35% hydrogen peroxide caused no alteration in the interface between enamel and composite resin, Tetric Ceram, fillings and the topical application of 35% hydrogen peroxide on enamel and composite resin, Tetric Ceram, caused an alteration of their surface topography, featuring a predominance of depressions after the bleaching treatment.