Dosagem da atividade da adenosina desaminase (ADA) / Adenosine deaminase activity (ADA) measurement

A atividade da adenosina desaminase (ADA) tem se mostrado útil para o diagnóstico da principal causa de derrame em nosso país, a tuberculose. Contudo, sua dosagem ainda não vem sendo utilizada de rotina. Acreditamos que a não existência de um kit comercial validado seja um dos principais fatores. Neste artigo descrevemos os cuidados com o líquido, a técnica de dosagem e o rendimento desta no diagnóstico da tuberculose de diversas localizações

What is the probability of a patient presenting a pleural effusion due to tuberculosis?

INTRODUCTION: In Rio de Janeiro, in almost half of the cases of pleural tuberculosis (PT) treatment begins without substantiation of diagnosis. We examined variables associated with this disease. METHOD: We studied 215 consecutive patients; 104 had tuberculosis (TB) and 111 did not (NTB): 41 neoplasms, 29 transudates, 28 para-pneumonic and 13 other etiologies. Clinical and laboratory variables were assessed in a combined manner using likelihood ratios (LR) and Bayes' theorem to determine the probability of PT. RESULTS: Among the variables examined, adenosine deaminase (ADA) levels, lymphocyte cell percentage, protein and age were the best indicators for the diagnosis of PT. Association of ADA with any of the other variables led to a LR+ higher than 10 and a LR- lower than 0.1, indicating the presence or absence of PT, with an individual probability of more than 90 percent or of less than 10 percent considering that there was a 50 percent initial probability associated with the presence of PT. CONCLUSIONS: Since ADA is highly sensitive, we can practically exclude TB as the cause of effusion when there are low ADA values. However, to confirm the possibility of TB we recommend that other variables, such as prevalence of lymphocytes (higher than 90 percent), and high protein levels (more than 4g/dL); low age (less than 45 years) also should be considered.