An Antimicrobial Activity of the Brown Seaweed Padina Tetrastromatica Extract in Different Concentration Against Human Pathogenic Bacteria.

The brown seaweed Padina tetrastromatica which can be collected from Gulf Of Mannar Sea shore Thoothukudi, India. In this present study was focused on the antimicrobial activity of the selected seaweed against six human pathogenic bacteria (Gram +ve: Staphylococcus aureus, Bacillus subtilis, Lactobacillus acidophilus and Gram -ve: Pseudomonas aeruginosa, Escherichia coli and Proteus mirabilis) by the agar well diffusion method. Here, different concentrations of the extract from Padina tetrastromatica were tested for probable antimicrobial activity and the extracts were prepared by five different solvents such as Acetone, Chloroform, Ethanol, Ethyl acetate and methanol. The experimental results shows that the highest antimicrobial activity 15mm was shown by Ethyl acetate extract against S. aureus and the lowest activity observed in methanol extract 1mm against E.coli. The experiment concludes that the extract of Ethyl acetate forms a good activity against all the six organisms.

Effect of probionts on the growth and food utilization of clown fish, amphiprion sebae (bleeker (1853)).

To evaluate the efficiency of different probionts (Lacto bacillus and yeast) on clown fish, Amphiprion sebae three different pelleted diets were formulated with 30% protein. Two diets were supplemented with probionts such as 2% Lacto bacillus and 2% yeast without probiont was used as control. The water stability of these diets was studied and the leaching percentage during 6 hrs period was 17.15 to 18.25%. The specific growth rate of control diet fed fish has the lowest value of 3.65 to 4% in probionts supplemented diets fed fishes. The gross production efficiency was also higher in probionts supplemented diet fed groups than control group.