Study of Correlation and Path Coefficient Analysis for Different Morphological Characters in Forage Sorghum (Sorghum bicolor L. Moench)

This research was conducted to investigate the correlation and path coefficient analysis of thirty three genotypes for green fodder yield and its attributes of forage sorghum. The experimental material was planted in Completely Randomized Block Design (RBD)in three replication during Kharif in the year 2019-20 at Crop Research Centre, SVPUA&T, Modipuram, Meerut - 250110 (U.P.). The study of association and path analysis evaluation carried out using the ten green fodder yield traits, viz., days to 50% flowering, plant height, leaf length, leaf breadth, leaf area, stem girth, leaves per plant, leaf stem ratio, total soluble solids and green fodder yield per plant. There is significant and positive correlation with green fodder yield per plant was exhibited with stem girth, leaves per plant, leaf length, leaf breadth, leaf stem ratio and total soluble solids at genotypic and phenotypic level. While leaf area showed the maximal direct effect on green fodder yield per plant followed by stem girth, leaves per plant and leaf stem ratio. There for selection for these traits is advisable for improving the character of green fodder yield.

Enhancing Growth and Yield of Chickpea (Cicer arietinum L.) Varieties through Foliar Application of Micronutrients under Field Condition

Extensively, it is imperative to address the pervasive issue of nutrient deficiencies among humans and animals in numerous underdeveloped regions. To tackle this challenge, a field experiment was conducted to investigate the impact of foliar applications of Zinc, Boron, and Iron on the growth, yield attributes, and overall yield of chickpea (Cicer arietinum L.) varieties during the Rabi seasons of both 2018-19 and 2019-20. The field experiment was meticulously designed using a Split Plot Design, wherein three distinct chickpea varieties (V1-KGD-1168, V2-Radhey, and V3-KWR-108) were assigned to the main plots. The subplots were dedicated to seven micronutrient treatment combinations, namely (M1-Control), (M2-Zinc @ 0.5%), (M3-Boron @ 0.2%), (M4-Iron @ 0.1%), (M5-Zinc @ 0.5% + Boron @ 0.2%), (M6-Zinc @ 0.5% + Iron @ 0.1%), and (M7: Zinc @ 0.5% + Boron @ 0.2% + Iron @ 0.1%). Consequently, a total of 21 treatment combinations were meticulously replicated three times. The findings of the experiment unveiled that the Radhey variety significantly influenced various parameters, including plant height, the number of branches per plant, fresh plant weight, 100-seed weight (17.21, 17.25g), seed yield (2118, 2228 kg ha-1), gross return (112396, 113628 INR ha-1), net return (83154, 83616 INR ha-1), and the benefit-to-cost ratio (B: C ratio) (2.84, 2.79) of chickpea for both the years 2018-19 and 2019-20, respectively. Regarding the micronutrient treatments, the foliar application of Zinc @ 0.5% + Boron @ 0.2% + Iron @ 0.1% exhibited a notably positive impact on plant height, the number of branches per plant, fresh plant weight, 100-seed weight (16.94, 16.97g), seed yield (2162, 2276 kg ha-1), gross return (114634, 116076 INR ha-1), net return (85041, 85712 INR ha-1), and B: C ratio (2.87, 2.82) of chickpea during both the years 2018-19 and 2019-20, respectively. Based on these compelling results, farmers are strongly advised to cultivate the Radhey variety of chickpea while implementing foliar applications of Zinc @ 0.5% + Boron @ 0.2% + Iron @ 0.1% for enhanced growth and higher yields.

Weed Dynamics of Fodder Maize as Influenced by Different Herbicides

The advancements in fodder maize cultivation practices over the past few years, has paved a change in the makeup of weed associations. This made it necessary to incorporate new, effective herbicides in the struggle against maize's primary weeds. Therefore, a field experiment was conducted at Research Farm, AICRP on Forage Crops, Department of Agronomy, JNKVV, Jabalpur (Madhya Pradesh) during Kharif season 2019 to devise an appropriate combination of herbicides required for the control of the complex weed diversity in fodder maize. The field experiment was laid out in randomized block design (RBD) with ten treatments (eight herbicidal combinations along with hand weeding twice at 20 and 40 DAS and a weedy check) and replicated thrice. Observations were recorded on the weed parameters and fodder yield of maize. Among monocots, the experimental field was infested with Echinochloa colona, Commelina communis and Digitaria sanguinalis. Phyllanthus niruri and Eclipta alba were dominated among dicots. Among all herbicidal treatment, topramezone 35 g/ha plus atrazine 250 g/ha was found to be significantly superior in reducing total weed density (9.49/m2, 11.50/m2) and total weed dry weight (6.43 g/m2, 7.91 g/m2) at 30 and 45 DAS, respectively and recorded the lowest weed index (1.82%). Also, the maximum green fodder yield (472.68 q/ha) was recorded in topramazone 35 g/ha + atrazine 250 g/ha compared to other treatments. Overall, this study indicated that the combination of topramazone 35 g/ha + atrazine 250 g/ha could be an alternative approach to hand weeding and alone herbicides in fodder maize.

Antioxidant and antiarthritic potential of berberine: In vitro and in vivo studies / 中草药·英文版

OBJECTIVE@#To extract and isolate berberine from Berberis aristata (Berberidaceae). Isolated berberine was characterised using spectroscopy and its antioxidant and antiarthritic activity was analyzed.@*METHODS@#The berberine was isolated from B. aristata using microwave-assisted extraction (MAE) and characterised by a spectroscopic technique. The isolated berberine was evaluated for its antioxidant activity in DPPH, nitric oxide, and superoxide scavenging assays, while antiarthritic activity was evaluated in the complete freund's adjuvant (CFA)-induced arthritis rat model.@*RESULTS@#The antioxidant activity of berberine revealed potent antioxidant activity in DPPH, nitric oxide, and superoxide scavenging assays. The in vivo antiarthritic activity of berberine in the CFA-induced arthritis rat model showed a significant reduction in paw diameter, arthritic score, and an increase in body weight. Furthermore, a concentration-dependent ameliorating action of berberine on haematological parameters was noticed. Proinflammatory biomarkers, including IL-6, IL-10, and TGF-b in serum were reported, and histopathology examination revealed that berberine decreased pannus formation, synovial hyperplasia, and bone erosion. Radiographic investigation showed soft tissue inflammation, bone resorption and erosion, joint gap reduction, and substantial connective tissue expansion after treatment with berberine.@*CONCLUSION@#The ameliorating action on haematological parameters and proinflammatory biomarkers of berberine makes them a suitable remedy for the treatment of arthritis.

A STUDY OF CLINICAL AND ETIOLOGICAL PATTERN AND SEASONAL VARIATION IN THE INCIDENCE OF ANTERIOR UVEITIS AT A TERTIARY CARE CENTER

Purpose: The aim was to study the various pattern and any seasonal variation in the incidence of anterior uveitis at a tertiary care center. An observational study was conducted on 100 patients of all Methods: new uveitis cases who attended the OPD between January 2020 to December 2021 of a tertiary care center, J.L.N. medical college and hospital, Ajmer, Rajasthan. We grouped the months into winter, autumn, monsoon, spring and summer according to Indian weather. Cases were screened along with detailed clinical and laboratory investigation to ?nd out there clinical etiological causes. 100 Patients of uveitis were examined. There was male predominance (66%) with male to female Result: ratio 1.94:1. We found cases with unilateral (91%) uveitis to be more common, with more Right eye (48%) involvement. Majority (40%) of cases remained idiopathic. HLA-B27 (37%) was commonest association followed by lens induced and Tuberculosis. The incidence of uveitis in winter season (38%) followed by 33% in transitional season and 29% in summer. Non- Conclusion: infectious uveitis were more common. In Non-infectious uveitis, most common entity found to be Idiopathic and HLA-B27 associated whereas in infectious uveitis, ocular tuberculosis was found to be the leading cause. Seasonally uveitis were more in winter and less in summer

Epidemiological scenario of leprosy in marginalized communities of India: Focus on scheduled tribes

The Scheduled Tribes (STs) are designated among the most disadvantaged social groups in India. Until the year 2005 (pre-elimination era of leprosy in India), several leprosy-specific control field programmes were implemented, which have been discontinued subsequently. Since then, leprosy diagnosis and treatment have been integrated with General Health Services. Thereafter, specialized expertise for the early diagnosis of leprosy has been gradually diminishing, especially at the peripheral clinics in remote areas. Hence, leprosy cases usually remain undetected for a long time and persist as endemic reservoirs. The tribal population of India accounts for just 8.6 per cent of the overall population. However, 18.5 per cent of the new leprosy cases were detected within the tribal community in the year 2020, indicating a disproportionately high burden of leprosy among the tribal population. Recent data suggest that these health disparities can be mainly related to the increased marginalization of STs as compared to other communities. This shows the need to further explore the current situation of leprosy in STs so that suitable interventions can address the contributing factors, leading to health inequalities in disadvantaged socio-economic groups. Therefore, this review aims to present the current distribution of leprosy in marginalized communities with a special emphasis on STs. Further, this review discusses how resources might be mobilized for such communities to find and treat undetected leprosy patients in STs to enable effective control of leprosy through early detection and timely treatment.

Integrative Approach in the Management of Janusandhivata w.s.r. to Osteoarthritis

Janusandhivata is a Vataj disorder in which Vata resides in Janu sandhi (Vimarga gaman) and produces various signs and symptoms according to its Gunas. In modern sciences Janusandhivata is correlated with osteoarthritis. In the present study integrative measures like allopathy medicines, Ayurvedic medicines and Karmas, yoga, naturopathy and exercises are studied for the effective relief of symptoms of Janu sandhigata vata i.e., OA. Integrative measures are studied to provide effective and fast relief to the patient. Modern treatment provides instant relief while on the other hand Ayurveda heals by curing the disease from root and is considered Apunarbhav chikitsa which means it prevents recurrence of the disease. Other measures aid in achieving the ultimate goal of cure from the disease.

Comparative Study of Tamsulosin versus Placebo in the Management of Lower Ureteric Stone

Aim: This study aims to compare the effect of tamsulosin versus placebo in the management of lower ureteric stones.Material and Methods: A total of 100 diagnosed patients of lower ureteric stone from the period of June 1, 2018, to May 31, 2019(12 months), were taken for the study. The study group was divided into three groups, Group A taken tamsulosin 0.4 mg + steroids(deflozacort 6 mg), Group B taken tamsulosin 0.4 mg + dicyclomine 20 mg, and Group C dicyclomine 20 mg alone. The results ofthree group were compared by expulsion rate and expulsion time of stones. Chi-square test was used for calculating the effect.Results: The mean age of Group A was 38.46 ± 10.68 years, Group B was 33.87 ± 9.62 years, and in Group C 32.94 ± 7.07 years.There was no significant relation between ages in stone expulsion. The mean calculus size in Group A was 5.93 ± 2.12 mm, Group Bwas 6.00 ± 2.03 mm, and Group C was 5.00 ± 2.01 mm. P = 0.784 was not statistically significant. In Group A, 40% of stones were onthe left side and 29.7% on the right side, in Group B, 20% of stones were on the left side and 40% on the right side, and in Group C,40% of stones were on the left side and 30.7% on the right side. P = 0.126 is not statistically significant. The mean time taken for stoneexpulsion in Group A was 5.39 ± 1.94 days, Group B was 4.75 ± 2.03 days, and Group C was 6.76 ± 1.90 days. P = 0.006 (<0.05)is highly statistically significant. Hence, in the present study, in Group A, 29 patients out of 33 patients, in Group B, 28 patients outof 33, and in Group C, 22 patients out of 34 patients, the stone was expelled out. P = 0.040 (<0.05) is highly statistically significant.There was no statistically significant difference which was found between the three groups in age, size, and side of stone.Conclusion: The results of this study have shown a potentially important role of tamsulosin for conservative expulsive therapyof distal ureteral stones, broadening pharmacological indications rather than endoscopic treatments for the resolution of thisdisease. Obviously, further studies with larger number of cases are necessary to validate these promising and statisticallysignificant results. The comparison with minimally invasive procedures in terms of cost and efficacy was useful, highlighting apredominant role of first-line pharmacological treatment, which can be easily be provided in an outpatient setting and not onlyat large, technologically advanced, centers. The drug tamsulosin proved to be safe and effective as demonstr ated by the lowincidence of side effects and the increased stone expulsion rate and reduced expulsion time. Moreover, medical expulsivetherapy with tamsulosin considerably decreased the analgesic use, thereby reducing additional need for pain relief and servedas an effective bridge between watch-and-wait management and surgical intervention.

Snake Venom Phospholipase A2 and its Natural Inhibitors

Snakebite is a severe medical, economic, and social problem across the world, mostly in the tropical and subtropical area. These regions of the globe have typical of the world's venomous snakes present where access to prompt treatment is limited or not available. Snake venom is a complex mixture of toxin proteins like neurotoxin and cardiotoxin, and other enzymes like phospholipase A2 (PLA2 ), haemorrhaging, transaminase, hyaluronidase, phosphodiesterase, acetylcholinesterase, cytolytic and necrotic toxins. Snake venom shows a wide range of biological effects like anticoagulation or platelet aggregation, hemolysis, hypotension and edema.Phospholipase A2 is the principal constituent of snake venom; it catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid, which is the precursor of eicosanoids including prostaglandins and leukotrienes. The information regarding the structure and function of the phospholipase A2enzyme may help in treating the snakebite victims. This review article constitutes a brief description of the structure, types, mechanism occurrence, and tests of phospholipase A 2 and role of components of medicinal plants used to inhibit phospholipase A2 .

Snake Venom Phospholipase A2 and its Natural Inhibitors

Snakebite is a severe medical, economic, and social problem across the world, mostly in the tropical and subtropical area. These regions of the globe have typical of the world's venomous snakes present where access to prompt treatment is limited or not available. Snake venom is a complex mixture of toxin proteins like neurotoxin and cardiotoxin, and other enzymes like phospholipase A2 (PLA2 ), haemorrhaging, transaminase, hyaluronidase, phosphodiesterase, acetylcholinesterase, cytolytic and necrotic toxins. Snake venom shows a wide range of biological effects like anticoagulation or platelet aggregation, hemolysis, hypotension and edema.Phospholipase A2 is the principal constituent of snake venom; it catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid, which is the precursor of eicosanoids including prostaglandins and leukotrienes. The information regarding the structure and function of the phospholipase A2enzyme may help in treating the snakebite victims. This review article constitutes a brief description of the structure, types, mechanism occurrence, and tests of phospholipase A 2 and role of components of medicinal plants used to inhibit phospholipase A2 .

Development of Protocol for Production of Primary Antibody against Ovalbumin Protein in Chicken for Detection of the Protein through Western Blotting

Ovalbumin, a major protein of egg white plays many roles including providing nutrition to the developing embryo, acting as coagulating agent, folliculogenesis and angiogenesis in chicken and other animals. This protein is expressed mainly in magnum and then deposited over the yolk of the oocyte/zygote. Hence, it is important in formation of egg and is an essential target to measure. We cloned chicken ovalbumin CDS in pAcGFP-C1 vector and has been initially expressed in chicken primary magnum cell culture. The ovalbumin protein tagged with 6x Histidine was purified from cell culture and used for production of primary antibody in rat. The ovalbumin protein along with freund’s adjuvant was injected to the rat, booster was given, and finally, hyper-immune sera was collected from rat. The antisera was purified for isolation of IgG. The IgG was used as primary antibody for Western blotting. Through Western blotting, ovalbumin protein isolated from chicken magnum was detected and the protocol was established to detect chicken ovalbumin protein.

Genetic diversity and population structure analysis of Asian and African aromatic rice (Oryza sativa L.) genotypes

Rice germplasms collected from different regions could be used as valuable resources for the future breeding programme. For the utilization of such collections, knowledge about the level and distribution of genetic diversity among these collections will facilitate the breeder. In this study, we report the phenotypic correlation, biochemical quality parameters and population genetic analysis of 35 rice accessions including 34 aromatic rice from different countries and a nonaromatic, Nagina 22, a well-known drought resistance variety. Further biochemical quality analysis, gel consistency test, molecular diversity analysis with 55 simple sequence repeat markers, population structure analysis and pair wise FST analysis were also conducted to assess the genetic diversity. The collected rice genotypes showed significant variability in different agronomic traits, i.e. spikelet per panicle, branches per panicle etc. Results obtained from the above tests demonstrated the importance of regional genetic studies for understanding the diversification of aromatic rice in Asian and African rice.

Differential Expression of Serum Lysozyme Allelic Variants in Muzaffarnagri Sheep

Lysozyme degrades the bacterial cell walls and gives rise to degradation product that stimulates and activates the immune system. Several gram positive and gram negative bacteria were found to be susceptible to different degree of purified lysozyme. Variation in promoter region may regulate the expression of a particular gene. Hence, considering lysozyme gene a potential marker for general immune response, expression pattern of various genotypes on the basis of variations in promoter region is investigated in Muzaffarnagri sheep. A 268 bp fragment spanning partial promoter, exon 1 and partial intron 1 of serum lysozyme gene were amplified and sequenced. Sequencing revealed five genotypes AA, AB, AC, BB and CC and consequently three alleles A, B and C in Muzaffarnagri sheep. Differential expression study of various genotypes by real time pcr revealed significant difference (P≤0.05) in the serum lysozyme expression in animals having different genotypes. Animals having AA genotype showed higher expression of serum lysozyme than the animals having AB, AC and BB genotype.

Assessment of Bacteriological Profile of Bile in Cholelithiasis Patients Undergoing Cholecystectomy

Background: Cholecystectomy is currently a frequentlyperformed operation. The most common reason for acholecystectomy is gallbladder stones. Hence we planned thepresent study to assess the bacteriological profile of bile incholelithiasis patients undergoing laparoscopiccholecystectomy (LC).Materials & Methods: A total of 100 patients scheduled toundergo LC were included in the present study. Demographicand clinical profile of all the patients was obtained. Completebiochemical and hematological profile of all the patients wasobtained. All the patients underwent LC under the hands ofskilled surgeons. Bile samples were collected and were sent tothe department of microbiology for further analysis.Results: Micro-organisms were found to be present in 28percent of the bile samples. Escherichia coli were the mostcommonly observed micro-organism found to be present in 17cases. Enterococcus spps. and Staphylococcus aureus werefound to be present in 4 and 3 cases respectively.No- significant results were obtained while correlating thepresence of micro-organisms with type of stone.Conclusion: Bile of the cholelithiasis patients undergoing LCoften show bactibilia.

The double burden of malnutrition among adults in India: evidence from the National Family Health Survey-4 (2015-16) / 한국역학회지

OBJECTIVES@#India still faces the burden of undernutrition and communicable diseases, and the prevalence of overweight/obesity is steadily increasing. The discourse regarding the dual burden of underweight and overweight/obesity has not yet been widely explored in both men and women. The present study assessed the determinants of underweight and overweight/obesity in India among adult men and women aged 15-49.@*METHODS@#Population-based cross-sectional and nationally representative data from the National Family Health Survey-4 (2015-16), consisting of a sample of men and women, were analyzed. Stratified 2-stage sampling was used in the NFHS-4 study protocol. In the present study, bivariate and adjusted multinomial logistic regression analyses were performed to determine the correlates of underweight and overweight/obesity.@*RESULTS@#The results suggested a persistently high prevalence of underweight coexisting with an increased prevalence of overweight/obesity in India. The risk of underweight was highest in the central and western regions and was also relatively high among those who used either smoking or smokeless tobacco. Overweight/obesity was more prevalent in urban areas, in the southern region, and among adults aged 35-49. Furthermore, level of education and wealth index were positively associated with overweight/obesity. More educated and wealthier adults were less likely to be underweight.@*CONCLUSIONS@#In India, underweight has been prevalent, and the prevalence of overweight/obesity is increasing rapidly, particularly among men. The dual burden of underweight and overweight/obesity is alarming and needs to be considered; public health measures to address this situation must also be adopted through policy initiatives.

The double burden of malnutrition among adults in India: evidence from the National Family Health Survey-4 (2015-16) / 한국역학회지

OBJECTIVES: India still faces the burden of undernutrition and communicable diseases, and the prevalence of overweight/obesity is steadily increasing. The discourse regarding the dual burden of underweight and overweight/obesity has not yet been widely explored in both men and women. The present study assessed the determinants of underweight and overweight/obesity in India among adult men and women aged 15-49.METHODS: Population-based cross-sectional and nationally representative data from the National Family Health Survey-4 (2015-16), consisting of a sample of men and women, were analyzed. Stratified 2-stage sampling was used in the NFHS-4 study protocol. In the present study, bivariate and adjusted multinomial logistic regression analyses were performed to determine the correlates of underweight and overweight/obesity.RESULTS: The results suggested a persistently high prevalence of underweight coexisting with an increased prevalence of overweight/obesity in India. The risk of underweight was highest in the central and western regions and was also relatively high among those who used either smoking or smokeless tobacco. Overweight/obesity was more prevalent in urban areas, in the southern region, and among adults aged 35-49. Furthermore, level of education and wealth index were positively associated with overweight/obesity. More educated and wealthier adults were less likely to be underweight.CONCLUSIONS: In India, underweight has been prevalent, and the prevalence of overweight/obesity is increasing rapidly, particularly among men. The dual burden of underweight and overweight/obesity is alarming and needs to be considered; public health measures to address this situation must also be adopted through policy initiatives.

Access to healthcare among the Empowered Action Group (EAG) states of India: Current status and impeding factors .

Background. Access to healthcare is crucial for meeting the health needs of Indians. We explored factors impeding access to public sources of healthcare among the Empowered Action Group (EAG) states of India. We also examined the extent to which Indians depend on public and private sources of healthcare in the EAG states. Methods. Our study is based on the unit-level records of 9988 ailing persons, who were surveyed among the EAG states in the 71stround of the National Sample Survey (NSS), conducted during January–June 2014 on the theme ‘Social consumption: Health’. To analyse the socioeconomic factors, we did logistic regression using STATA version 12.0. Results. Despite a vast public health infrastructure in the EAG states, around three-fourths of inpatients are dependent on private sources of healthcare in both rural (70%) and urban (78%) areas. Poor quality and long waiting time in accessing healthcare from public health facilities remain big concerns for inpatients of the EAG states. Conclusion. To make public health services more accessible, there is a need to improve the quality of services, enlarge infrastructure to reduce waiting time, and enhance the physical reach to inpatients in the EAG states of India. Public health services will then be able to compete with those in the private sector. Natl Med J India 2016;29:267–73

Novel approach of aceclofenac fast dissolving tablet

Fast disintegrating tablets [FDTs] have received ever increasing demand during the last decade, and the field has become a hastily growing area in the pharmaceutical industry. Upon introduction into the mouth, these tablets dissolve or disintegrate in the mouth in the absence of additional water for easy administration of active pharmaceutical ingredients. Aceclofenac, an NSAID, has been recommended orally for the treatment of bone and connective tissue disorder and thus the formulation of the same resulted in development of several FDT technologies. The present aim is to formulate a tablet which disintegrate and dissolve rapidly and give its rapid onset of action: analgesic, antipyretic and anti-inflammatory action. Besides, the conventional tablets also show poor patient compliance an attempt had been made to formulate for FDT of aceclofenac by using various super disintegrants like sodium starch glycolate, croscarmellose sodium and crosspovidone [polyplasdone XL] and PEG 6000 followed by novel technique. The tablets were evaluated for friability, hardness, weight variation, disintegration time, wetting time, in vitro dissolution studies and drug content studies. It was concluded that the batch which was prepared by using combination of crosspovidone and sodium starch glycolate as a super disintegrant shows excellent disintegration time, enhance dissolution rate, taste masking and hence lead to improve efficacy and bioavailability of drug