RESUMO
The killer toxin of Hansenula anomala showed killer activity toward some different microrganisms. The killer toxin produced by this species was purified. This toxin inhibited completely the growth of 2 species of yeasts,. Candida albicans, and CKefyr; three species of dermatophytes, Microsporum gypseum, M. Canis and Trichophyton mentagrophytes; and 2 species of moulds Fusarium solani and Aspergillus terreus tested at concentrations 100 ul/ml or less. The minimum inhibitory concentration [MIC] of the killer toxin against Candida guilliermondii and C. parapsilosis tested was 20 ul/ml or more. [MIC] of the killer toxin against, M. audoiunii, Sepedomium chrysospermun and Penicillium Coryophilum tested was 60 ul/ml or more. Finally the [MIC] of this killer toxin against the following bacterial strains: Proteus Vulgaris. Staphylococcus aureus, Salmonella typh, E. coli and Rhizobium legumnosarum tested was 25 ul/ml or more. But the [MIC] of this toxin against the two species of: Bacillus subtilus and Pseudomonas areuginosa was 50 ul/ml or more. In this paper we found that the purified H. anomala killer toxin used for the determination the [MIC] of a wide range of yeasts, moulds and bacteria including a number of important species