Expression difference of toll-like receptors among chronic rhinosinusitis and nasal polyps / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To compare the expression difference of Toll-like receptor-2 (TLR-2) and Toll-like receptor-4 (TLR-4) protein and mRNA among the chronic rhinosinusitis tissues, nasal polyps tissues, and normal mucosa tissues.</p><p><b>METHODS</b>The mRNA expression of TLR-2 and TLR-4 in chronic rhinosinusitis tissues from 10 patients, in nasal polyp tissues from 10 patients, and in inferior turbinate tissues from 10 patients underwent nasal septum operation was detected by means of reverse transcriptase-polymerase chain reaction (RT-PCR). Immunohistochemistry (IHC) was used to detected the expression of TLR-2 and TLR-4 protein in a different set of 20 chronic rhinosinusitis tissues, 20 nasal polyp tissues, and 20 normal inferior turbinate tissues.</p><p><b>RESULTS</b>(1) The mRNA and protein expression of TLR-2 and TLR-4 was detected on epithelial and glandular cells membrane in all chronic rhinosinusitis, nasal polyps and control tissues . (2) The mRNA and protein expression of TLR-2 and the mRNA expression of TLR-4 in chronic rhinosinusitis tissues was significantly increased compared with that in nasal polyps and control tissues (P < 0.05). (3) The expression intensity of TLR-2 and TLR-4 mRNA and protein between nasal polyps and control tissues was found no significant difference (P > 0.05).</p><p><b>CONCLUSIONS</b>Different TLR-2 and TLR-4 protein and mRNA level in chronic rhinosinusitis and nasal polyp tissues might imply that TLR-2 and TLR-4 play different role in the pathogenesis of chronic rhinosinusitis and nasal polyps.</p>

Relationship between the expression of tenascin C and TGF-beta1 in human nasal polyp tissues / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To explore the mechanism underlying the increased expression of tenascin C (TNC) in human nasal polyp tissues.</p><p><b>METHODS</b>The method of immunohistochemistry was used to detect the protein expression of TNC and transforming growth factor-beta1 (TGF-beta1) and their relationship in nasal polyp tissues. The cell culture, real-time RT-PCR and in situ ELISA techniques were employed to investigate the effect of TGF-beta1 and eosinophils on the TNC mRNA and protein expression in BEAS-2B immortalized human bronchial epithelial cells.</p><p><b>RESULTS</b>(1) TNC and TGF-beta1 protein expression were up-regulated in nasal polyp tissues. TNC expression level was associated with the number of TGF-beta1 positive cells (r = -0.58, P < 0.01) and TGF-beta1 positive eosinophils (r = -0.61, P < 0.01); (2) 1 ng/ml and 10 ng/ml TGF-beta1 induced TNC mRNA expression by 7.20 +/- 3.43-fold and 22.48 +/- 5.35-fold (P < 0.01) in BEAS-2B cells after 4 h stimulation respectively. The fluorescence intensity of TNC protein expression was 129. 50 +/- 47.42 and 151.20 +/- 48.36 after 24 h stimulation respectively. The protein expression was significantly increased compared with that without stimulation (60.60 +/- 38.53, P < 0.05); (3) Coculture BEAS-2B cells and eosinophils at 2:1, 1:1 and 1:2 ratio, TNC mRNA expression was induced by 4.90 +/- 1.40-fold, 5.48 +/- 1.60-fold and 4.78 +/- 1.32-fold (P < 0.01) in BEAS-2B cells after 4 h coculture respectively. The fluorescence intensity of TNC protein expression was 128.75 +/- 44.15, 142.33 +/- 29.06 and 131.33 +/- 20.87 after 24 h coculture respectively. The protein expression was significantly increased compared with that without eosinophils coculture (59.40 +/- 10.14, P < 0.05). Treatment with neutralizing antibody to TGF-beta1 significantly inhibited eosinophil-induced BEAS-2B cells TNC expression in a dose-dependent manner (P < 0.05).</p><p><b>CONCLUSION</b>TGF-beta1 and eosinophils can induce TNC expression in airway epithelial cells. The effect of eosinophils is partially mediated through TGF-beta1. Up-regulated expression of TNC in nasal polyp tissues is related to eosinophil-derived TGF-beta1.</p>