Clinical application value of soluble co-stimulatory molecule B7-H1 detection in colorectal cancer / 中华检验医学杂志

Objective To determine the level of soluble B7-H1 (sB7-H1) in serum of patients with colorectal cancer ( CRC) , and to investigate its clinical application value in CRC .Methods 152 cases of CRC, 57 cases of benign colorectal diseases and 59 healthy subjects were enrolled .ELISA was used to determine the sB7-H1 level in serum.The levels of carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9) and carbohydrate antigen 72-4 (CA72-4) were determined by electrochemiluminescence. The level of carbohydrate antigen 50 (CA-50) was determined by chemiluminescence.ROC curve was used to evaluate the diagnostic efficiency of sB 7-H1 alone or combined with other tumor markers in CRC .It also analyzed the correlation between serum sB 7-H1 level and some clinicopathological characteristics including tumor location , depth of invasion , lymph node metastasis status , distant metastasis status and tumor stages.Results (1)The differences of sB7-H1 level among CRC group, colorectal benign disease group and healthy control group ( compared with each other respectively ) were statistically significant ( P<0.001 ) . Levels of CEA, CA19-9, CA72-4 and CA-50 were significantly different between CRC group and benign colorectal disease group ( P<0.05 ) , while there was no significant difference between benign colorectal disease group and healthy control group (P>0.05).(2)The diagnosis ability of sB7-H1, CEA, CA19-9,CA72-4, and CA-50 were determined by the area under curve (AUC), which was 0.730, 0.772, 0.639, 0.663 and 0.635, respectively.The combination of sB7-H1 and CEA showed the best effect , and the AUC reached 0.831.(3)There was no significant correlation between the levels of sB 7-H1 and CEA, or CA19-9, CA72-4, CA-50.(4) Immunohistochemistry result showed that the positive rate of CRC group was 52％, while that of benign disease group was 6.3％.The difference of the positive rate between two groups was statistically significant (P<0.01).And there was a significant correlation between the positive rate of sB 7-H1 level in serum and tumor tissues of CRC patients ( P<0.05 ) .( 5 ) The serum sB7-H1 level in CRC patients was related to lymph node metastasis status (P<0.05).But there was no relationship between sB7-H1 level and other clinicopathological characteristics ( including tumor location , depth of invasion , distant metastasis status and tumor stage ) (P>0.05).Conclusion Serum sB7-H1 has a high clinical application value and could serve as a new tumor marker in CRC .

Study on objectively evaluating skin aging according to areas of skin texture / 生物医学工程学杂志

Skin aging principles play important roles in skin disease diagnosis, the evaluation of skin cosmetic effect, forensic identification and age identification in sports competition, etc. This paper proposes a new method to evaluate the skin aging objectively and quantitatively by skin texture area. Firstly, the enlarged skin image was acquired. Then, the skin texture image was segmented by using the iterative threshold method, and the skin ridge image was extracted according to the watershed algorithm. Finally, the skin ridge areas of the skin texture were extracted. The experiment data showed that the average areas of skin ridges, of both men and women, had a good correlation with age (the correlation coefficient r of male was 0.938, and the correlation coefficient r of female was 0.922), and skin texture area and age regression curve showed that the skin texture area increased with age. Therefore, it is effective to evaluate skin aging objectively by the new method presented in this paper.

Efffect of baicalin on antipyresis and influence on cytokine / 中国中药杂志

<p><b>OBJECTIVE</b>To study the antipyretic effect of baicalin in inhibiting yeast-induced fever in rats and the influence on inflammatory cytokine, then explore the possible mechanism of baicalin in inhibiting yeast-induced fever in rats.</p><p><b>METHODS</b>Rat modles of pyrexia were established by subcutaneous injection of yeast (2 g x kg(-1)); the rats of were divided into the normal control, model, baicalin high, medium and low-dose group and the effect of baicalin on the changes of the rats' temperature were observed. Dual antibody ELISA method was used to test the changes of IL-6, IL-1beta and TNF-alpha contents in in serum , hypothalamus and cerebral spinal fluid (CSF). Then analyze the correlation between the inhibition ratio of temperature heighten on three different dose of baicalin and the inhibition ratio of the contents heighten on IL-6, IL-1beta and TNF-alpha.</p><p><b>RESULT</b>The high dose of baicalin significantly inhibited the yeast-induced fever of rats, and decresesed IL-6, IL-1beta and TNF-alpha contents in serum, hypothalamus and CSF. The inhibition ratio of temperature heighten of baicalin had direct correlation with the inhibition ratio of the heighten on IL-1beta content in serum, hypothalamus and CSF (r = 0.873, P < 0.05), also dose TNF-alpha (r = 0.862, P < 0.01).</p><p><b>CONCLUSION</b>Baicalin may have obvious antipyretic effect by decreasing the increasing contents of IL-1beta and TNF-alpha in rats.</p>