RESUMO
<p><b>OBJECTIVE</b>To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis.</p><p><b>METHOD</b>The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol- 0.1 mol x L(-1) phosphate buffer (pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the counter electrode was Pt platmun.</p><p><b>RESULT</b>The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and ( - ) -epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%, respectively.</p><p><b>CONCLUSION</b>The method can be used to control the quality of Rhizoma fagopyri dibotoryis.</p>
Assuntos
Benzaldeídos , Catequina , Catecóis , Cromatografia Líquida de Alta Pressão , Métodos , Medicamentos de Ervas Chinesas , Padrões de Referência , Eletroquímica , Fagopyrum , Química , Ácido Gálico , Hidroxibenzoatos , Plantas Medicinais , Química , Controle de Qualidade , Reprodutibilidade dos Testes , Rizoma , QuímicaRESUMO
<p><b>OBJECTIVE</b>To establish a HPLC fingerprint for quantitative analysis of the active constituents of jizhi syrup.</p><p><b>METHOD</b>HPLC analysis was performed on a Zorbax Sb C18 column (4.6 mm x 250 mm, 5 microm), the mobile phase in gradient elution was composed of (A) 1% acetic acid solution (including 0.2% triethylamine) and (B) acetonitrile, at a flow rate of 1.0 mL x min(-1). The column temperature was 30 degrees C and the wavelength was 280 nm.</p><p><b>RESULT</b>21 common peaks were tested in 10 batches of samples. Compared with the standard fingerprint, the similarity of each sample was greater than 0.99. At the same time, protocatechuic acid, protocatechu aldehyde, chlorogenic acid, caffeic acid, naringin and neohesperidin were found in all samples.</p><p><b>CONCLUSION</b>The established method can be used for the quality control of jizhi syrup.</p>